JBO Letters

Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy

[+] Author Affiliations
Shuo Tang, Tatiana B. Krasieva

University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, California 92617

Zhongping Chen

University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, and Department of Biomedical Engineering, Irvine, California 92617

Gabriel Tempea

Femtolasers Produktions GmbH, A-1100 Vienna, Austria

Bruce J. Tromberg

University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, and Department of Biomedical Engineering, Irvine, California 92617

J. Biomed. Opt. 11(2), 020501 (March 22, 2006). doi:10.1117/1.2177676
History: Received October 11, 2005; Revised January 17, 2006; Accepted January 17, 2006; Published March 22, 2006
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We have developed a multiphoton microscopy (MPM) system using a 12-fs Ti:sapphire laser with adjustable dispersion precompensation in order to examine the impact of pulse duration on nonlinear optical signals. The efficiencies of two-photon-excited fluorescence (TPEF) and second harmonic generation (SHG) were studied for various pulse durations, measured at the sample, ranging from 400fs to sub-20 fs. Both TPEF and SHG increased proportionally to the inverse of the pulse duration for the entire tested range. Because of improved signal-to-noise ratio, sub-20-fs pulses were used to enhance MPM imaging depth by approximately 160%, compared to 120-fs pulses, in human skin.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Shuo Tang ; Tatiana B. Krasieva ; Zhongping Chen ; Gabriel Tempea and Bruce J. Tromberg
"Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy", J. Biomed. Opt. 11(2), 020501 (March 22, 2006). ; http://dx.doi.org/10.1117/1.2177676


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