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Special Section on Cardiovascular Photonics

Detection of morphological markers of vulnerable atherosclerotic plaque using multimodal spectroscopy

[+] Author Affiliations
Obrad R. Šćepanović

Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139

Maryann Fitzmaurice

University Hospitals of Cleveland, and Case Western Reserve University, Cleveland, Ohio

Joseph A. Gardecki, George O. Angheloiu, Samir Awasthi

Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139

Jason T. Motz

Massachusetts General Hospital, Wellman Center for Photomedicine, Boston, Massachusetts

John R. Kramer, Ramachandra R. Dasari, Michael S. Feld

Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139

J. Biomed. Opt. 11(2), 021007 (March 27, 2006). doi:10.1117/1.2187943
History: Received July 02, 2005; Revised November 29, 2005; Accepted December 22, 2005; Published March 27, 2006
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Vulnerable plaques, which are responsible for most acute ischemic events, are presently invisible to x-ray angiography. Their primary morphological features include a thin or ulcerated fibrous cap, a large necrotic core, superficial foam cells, and intraplaque hemorrhage. We present evidence that multimodal spectroscopy (MMS), a novel method that combines diffuse reflectance spectroscopy (DRS), intrinsic fluorescence spectroscopy (IFS), and Raman spectroscopy (RS), can detect these markers of plaque vulnerability. To test this concept, we perform an MMS feasibility study on 17 human carotid artery specimens. Following the acquisition of spectra, each specimen is histologically evaluated. Two parameters from DRS, hemoglobin concentration and a scattering parameter, are used to detect intraplaque hemorrhage and foam cells; an IFS parameter that relates to the amount of collagen in the topmost layers of the tissue is used to detect the presence of a thin fibrous cap; and an RS parameter related to the amount of cholesterol and necrotic material is used to detect necrotic core. Taken together, these spectral parameters can generally identify the vulnerable plaques. The results indicate that MMS provides depth-sensitive and complementary morphological information about plaque composition. A prospective in vivo study will be conducted to validate these findings.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Obrad R. Šćepanović ; Maryann Fitzmaurice ; Joseph A. Gardecki ; George O. Angheloiu ; Samir Awasthi, et al.
"Detection of morphological markers of vulnerable atherosclerotic plaque using multimodal spectroscopy", J. Biomed. Opt. 11(2), 021007 (March 27, 2006). ; http://dx.doi.org/10.1117/1.2187943


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