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Special Section on Cardiovascular Photonics

Bioenzymatic detection of troponin C using micro-opto-electro-mechanical systems

[+] Author Affiliations
Jeetender Amritsar, Ion Stiharu, Muthukumaran Packirisamy

Concordia University, Micromechatronics Laboratory, Concave Research Center, 1455 de Maisonneuve Boulevard, Montreal, Quebec H3G 1M8 Canada

J. Biomed. Opt. 11(2), 021010 (June 30, 2005December 02, 2005December 05, 2005April 03, 2006). doi:10.1117/1.2186326
History: Received June 30, 2005; Revised December 02, 2005; Accepted December 05, 2005; Published April 03, 2006
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Diagnosis and monitoring of critical diseases such as acute myocardial infarction (AMI) require a quantitative analysis of biological molecules. A high-throughput identification of these biological molecules can be generated by using micro-electro-mechanical systems (MEMS) structures like simple cantilever beams, which respond to the intermolecular forces resulting from binding these molecules. Biochemical markers like troponin C are considered the primary markers for myocardial injury and have generated considerable interest. A 26-residue lytic membrane protein of bee venom melittin (ME) is chosen to interact with rabbit skeletal muscle troponin C (TnC) on the surface of the cantilever beams. An optical beam deflection method is employed to identify the enzymatic reaction on the surface of the cantilever due to these proteins. Identification of these proteins is also done using fluorescence spectroscopy (FS) to compliment the optical monitored deflection method. A second set of proteins like horse raddish peroxide (HRP) and hydrogen peroxide (H2O2) are applied to atomic force microscopy (AFM) cantilever beams to study their behavior under the enzymatic reactions of proteins. Identification of these proteins is done using Fourier transform infrared spectroscopy (FTIR). An analytical model of the cantilever beam is developed, and its mode shapes are studied by employing orthogonal polynomials in the classic Rayleigh-Ritz method. The surface stress caused by the enzymatic reaction of the proteins that leads to pure bending on the top surface of the cantilever is evaluated. The information provided by the experimental and analytical modeling reported in this work will be useful in the development of a portable biosensor for the detection of AMI.

© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Jeetender Amritsar ; Ion Stiharu and Muthukumaran Packirisamy
"Bioenzymatic detection of troponin C using micro-opto-electro-mechanical systems", J. Biomed. Opt. 11(2), 021010 (June 30, 2005December 02, 2005December 05, 2005April 03, 2006). ; http://dx.doi.org/10.1117/1.2186326


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