Special Section on Cardiovascular Photonics

Fluorophore-mediated, fiber-optic, multi-analyte, immunosensing system for rapid diagnosis and prognosis of cardiovascular diseases

[+] Author Affiliations
Liang Tang, Yongjie Ren, Bin Hong, Kyung A. Kang

University of Louisville, Speed School of Engineering, Department of Chemical Engineering, Louisville, Kentucky 40292

J. Biomed. Opt. 11(2), 021011 (April 17, 2006). doi:10.1117/1.2192529
History: Received July 02, 2005; Revised February 03, 2006; Accepted February 28, 2006; Published April 17, 2006
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A prototype of a fiber-optic, multi-analyte, immunobiosensing system was developed to simultaneously quantify disease-representing biomarkers in blood plasma. This system was for simultaneous quantification of two different groups of multi-biomarkers related to cardiovascular diseases (CVD): anticoagulants (protein C, protein S, antithrombin III, and plasminogen) for deficiency diagnosis; and cardiac markers (B-type natriuretic peptide, cardiac troponin I, myoglobin, and C-reactive protein) for coronary heart disease diagnosis. As an initial effort towards the development of a disposable and easy-to-use sensing cartridge as a rapid diagnostic tool for CVD related diseases, a prototype of a flow control system was also developed to automatically perform simultaneous four-analyte quantification. Currently, the system is capable of quantifying the multiple anticoagulants in their clinically significant sensing ranges within 5 minutes, at an average signal-to-noise (S∕N) ratio of 25. A simultaneous assay of the four cardiac markers can be performed within 10 min, at an average S∕N ratio of 20. When this highly portable multi-analyte sensing system is completed and successfully tested for CVD patient’s plasma, it can provide rapid (<10min) and reliable diagnostic and prognostic information at a patient’s bedside.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Liang Tang ; Yongjie Ren ; Bin Hong and Kyung A. Kang
"Fluorophore-mediated, fiber-optic, multi-analyte, immunosensing system for rapid diagnosis and prognosis of cardiovascular diseases", J. Biomed. Opt. 11(2), 021011 (April 17, 2006). ; http://dx.doi.org/10.1117/1.2192529


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