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Special Section on Cardiovascular Photonics

Three-dimensional optical coherence tomography of the embryonic murine cardiovascular system

[+] Author Affiliations
Wei Luo, Daniel L. Marks

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, Urbana, Illinois 61801

Tyler S. Ralston

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, Department of Electrical and Computer Engineering, Urbana, Illinois 61801

Stephen A. Boppart

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, Departments of Electrical and Computer Engineering, Bioengineering, and Medicine, Urbana, Illinois 61801

J. Biomed. Opt. 11(2), 021014 (April 18, 2006). doi:10.1117/1.2193465
History: Received June 01, 2005; Revised September 18, 2005; Accepted September 26, 2005; Published April 18, 2006
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Optical coherence tomography (OCT) is an emerging high-resolution real-time biomedical imaging technology that has potential as a novel investigational tool in developmental biology and functional genomics. In this study, murine embryos and embryonic hearts are visualized with an OCT system capable of 2μm axial and 15μm lateral resolution and with real-time acquisition rates. We present, to our knowledge, the first sets of high-resolution 2- and 3-D OCT images that reveal the internal structures of the mammalian (murine) embryo (E10.5) and embryonic (E14.5 and E17.5) cardiovascular system. Strong correlations are observed between OCT images and corresponding hematoxylin- and eosin-stained histological sections. Real-time in vivo embryonic (E10.5) heart activity is captured by spectral-domain optical coherence tomography, processed, and displayed at a continuous rate of five frames per second. With the ability to obtain not only high-resolution anatomical data but also functional information during cardiovascular development, the OCT technology has the potential to visualize and quantify changes in murine development and in congenital and induced heart disease, as well as enable a wide range of basic in vitro and in vivo research studies in functional genomics.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Wei Luo ; Tyler S. Ralston ; Stephen A. Boppart and Daniel L. Marks
"Three-dimensional optical coherence tomography of the embryonic murine cardiovascular system", J. Biomed. Opt. 11(2), 021014 (April 18, 2006). ; http://dx.doi.org/10.1117/1.2193465


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