Research Papers

Collagen birefringence in skin repair in response to red polarized-laser therapy

[+] Author Affiliations
Daniela de Fátima Teixeira da Silva

IPEN-CNEN/SP ,Instituto de Pesquisas Energéticas e Nucleares-Comissão Nacional de Energia Nuclear/SP, Centro de Lasers e Aplicações, CEP 05508-000, São Paulo, São Paulo, Brazil

Benedicto de Campos Vidal

IB-UNICAMP,Instituto de Biologia, Universidade Estadual de Campinas, CEP 13084-971, Campinas, São Paulo, Brazil

Denise Maria Zezell

IPEN-CNEN/SP,Instituto de Pesquisas Energéticas e Nucleares-Comissão Nacional de Energia Nuclear/SP, Centro de Lasers e Aplicações, CEP 05508-000, São Paulo, São Paulo, Brazil

Telma Maria Tenório Zorn

ICB-USP,Instituto de Ciências Biomédicas, Universidade de São Paulo, CEP 05508-000, São Paulo, S ão Paulo, Brazil

Silvia Cristina Núñez, Martha Simões Ribeiro

IPEN-CNEN/SP,Instituto de Pesquisas Energéticas e Nucleares-Comissão Nacional de Energia Nuclear/SP, Centro de Lasers e Aplicações, CEP 05508-000, São Paulo, São Paulo, Brazil

J. Biomed. Opt. 11(2), 024002 (March 22, 2006). doi:10.1117/1.2187418
History: Received August 16, 2005; Revised December 01, 2005; Accepted December 17, 2005; Published March 22, 2006
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We use the optical path difference (OPD) technique to quantify the organization of collagen fibers during skin repair of full-thickness burns following low-intensity polarized laser therapy with two different polarization incidence vectors. Three burns are cryogenerated on the back of rats. Lesion L is irradiated using the electric field vector of the polarized laser radiation aligned in parallel with the rat’s occipital-caudal direction. Lesion L is irradiated using the electric field vector of the polarized laser radiation aligned perpendicularly to the aforementioned orientation. Lesion C is untreated. A healthy area labeled H is also evaluated. The tissue samples are collected and processed for polarized light microscopy. The overall finding is that the OPD for collagen fibers depends on the electric field vector of the incident polarized laser radiation. No significant differences in OPDs are observed between L and H in the center, sides, and edges of the lesion. Lesions irradiated using the electric field vector of the polarized laser radiation aligned in parallel with the rat’s occipital-caudal direction show higher birefringence, indicating that collagen bundles in these lesions are more organized.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Daniela de Fátima Teixeira da Silva ; Benedicto de Campos Vidal ; Denise Maria Zezell ; Telma Maria Tenório Zorn ; Silvia Cristina Núñez, et al.
"Collagen birefringence in skin repair in response to red polarized-laser therapy", J. Biomed. Opt. 11(2), 024002 (March 22, 2006). ; http://dx.doi.org/10.1117/1.2187418


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