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Research Papers

Doppler flow imaging of cytoplasmic streaming using spectral domain phase microscopy

[+] Author Affiliations
Michael A. Choma, Audrey K. Ellerbee, Siavash Yazdanfar, Joseph A. Izatt

Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708

J. Biomed. Opt. 11(2), 024014 (April 10, 2006). doi:10.1117/1.2193167
History: Received April 19, 2005; Revised November 13, 2005; Accepted November 14, 2005; Published April 10, 2006
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Spectral domain phase microscopy (SDPM) is a function extension of spectral domain optical coherence tomography. SDPM achieves exquisite levels of phase stability by employing common-path interferometry. We discuss the theory and limitations of Doppler flow imaging using SDPM, demonstrate monitoring the thermal contraction of a glass sample with nanometer per second velocity sensitivity, and apply this technique to measurement of cytoplasmic streaming in an Amoeba proteus pseudopod. We observe reversal of cytoplasmic flow induced by extracellular CaCl2, and report results that suggest parabolic flow of cytoplasm in the A. proteus pseudopod.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Michael A. Choma ; Audrey K. Ellerbee ; Siavash Yazdanfar and Joseph A. Izatt
"Doppler flow imaging of cytoplasmic streaming using spectral domain phase microscopy", J. Biomed. Opt. 11(2), 024014 (April 10, 2006). ; http://dx.doi.org/10.1117/1.2193167


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