Research Papers

Real-time optoacoustic monitoring of vascular damage during photodynamic therapy treatment of tumor

[+] Author Affiliations
Liangzhong Xiang, Da Xing, Huaimin Gu, Diwu Yang, Sihua Yang, Lvming Zeng, Wei R. Chen

South China Normal University, MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, Guangzhou 510631 China

J. Biomed. Opt. 12(1), 014001 (February 02, 2007). doi:10.1117/1.2437752
History: Received July 28, 2006; Revised October 04, 2006; Accepted October 22, 2006; Published February 02, 2007
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The optoacoustic technique is a noninvasive imaging method with high spatial resolution. It potentially can be used to monitor anatomical and physiological changes. Photodynamic therapy (PDT)-induced vascular damage is one of the important mechanisms of tumor destruction, and real-time monitoring of vascular changes can have therapeutic signicance. A unique optoacoustic system is developed for neovascular imaging during tumor phototherapy. In this system, a single-pulse laser beam is used as the light source for both PDT and for concurrently generating ultrasound signals for optoacoustic imaging. To demonstrate its feasibility, this system is used to observe vascular changes during PDT treatment of chicken chorioallantoic membrane (CAM) tumors. The photosensitizer used in this study is protoporphyrin IX (PpIX) and the laser wavelength is 532nm. Neovascularization in tumor angiogenesis is visualized by a series of optoacoustic images at different stages of tumor growth. Damage of the vascular structures by PDT is imaged before, during, and after treatment. Rapid, real-time determination of the size of targeted tumor blood vessels is achieved, using the time difference of positive and negative ultrasound peaks during the PDT treatment. The vascular effects of different PDT doses are also studied. The experimental results show that a pulsed laser can be conveniently used to hybridize PDT treatment and optoacoustic imaging and that this integrated system is capable of quantitatively monitoring the structural change of blood vessels during PDT. This method could be potentially used to guide PDT and other phototherapies using vascular changes during treatment to optimize treatment protocols, by choosing appropriate types and doses of photosensitizers and doses of light.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Liangzhong Xiang ; Da Xing ; Huaimin Gu ; Diwu Yang ; Sihua Yang, et al.
"Real-time optoacoustic monitoring of vascular damage during photodynamic therapy treatment of tumor", J. Biomed. Opt. 12(1), 014001 (February 02, 2007). ; http://dx.doi.org/10.1117/1.2437752


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