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Research Papers

In vivo monitoring of singlet oxygen using delayed chemiluminescence during photodynamic therapy

[+] Author Affiliations
Yanchun Wei, Jing Zhou, Da Xing

South China Normal University, MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, Guangzhou 510631 China

Qun Chen

University of Colorado at Denver and Health Sciences Center, Department of Radiation Oncology, Aurora, Colorado 80262 and South China Normal University, MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, Guangzhou 510631 China

J. Biomed. Opt. 12(1), 014002 (February 02, 2007). doi:10.1117/1.2437151
History: Received September 06, 2006; Revised October 27, 2006; Accepted November 09, 2006; Published February 02, 2007
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It is known that singlet oxygen (O21) is the main factor mediating cytotoxicity in photodynamic therapy (PDT). The effectiveness of a PDT treatment is directly linked to the O21 produced in the target. Although the luminescence from O21 is suggested as an indicator for evaluating photodynamic therapy, the inherent disadvantages limit its potential for in vivo applications. We have previously reported that chemiluminescence can be used to detect O21 production in PDT and have linked the signal to the cytotoxicity. We further our investigation for monitoring O21 production during PDT. The lifetime of 3,7-dihydro-6-{4-[2-(N-(5-fluoresceinyl)thioureido)eth-oxy]phenyl}-2-methylimidazo {1,2-a} pyrazin-3-one-chemilumines-cence (FCLA-CL) is evaluated, and the results show that it is much longer than that of direct luminescence of O21. A gated measurement algorithm is developed to fully utilize the longer lifetime for a clean measurement of the CL without the interference from the irradiation light. The results show that it is practically feasible to use the technique to monitor the O21. Compared to the direct O21 luminescence measurement, our new technique is sensitive and can be realized with a conventional optical detector with excellent signal-to-noise ratio. It thus provides a means for real-time in vivo monitoring of O21 production during PDT.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Yanchun Wei ; Jing Zhou ; Da Xing and Qun Chen
"In vivo monitoring of singlet oxygen using delayed chemiluminescence during photodynamic therapy", J. Biomed. Opt. 12(1), 014002 (February 02, 2007). ; http://dx.doi.org/10.1117/1.2437151


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