Research Papers

Visualization of hepatobiliary excretory function by intravital multiphoton microscopy

[+] Author Affiliations
Yuan Liu

National Taiwan University, Department of Physics, Taipei 106 Taiwan

Hsiao-Ching Chen, Shu-Mei Yang

National Taiwan University Hospital and National Taiwan University, College of Medicine, Department of Internal Medicine, Taipei 100 Taiwan

Tzu-Lin Sun, Wen Lo

National Taiwan University, Department of Physics, Taipei 106 Taiwan

Ling-Ling Chiou, Guan Tarn Huang

National Taiwan University Hospital and National Taiwan University, College of Medicine, Department of Internal Medicine, Taipei 100 Taiwan

Chen-Yuan Dong

National Taiwan University, Department of Physics, Taipei 106 Taiwan

Hsuan-Shu Lee

National Taiwan University, College of Bio-Resources and Agricultural Institute of Biotechnology, Taipei 106 Taiwan and National Taiwan University Hospital and National Taiwan University, College of Medicine, Department of Internal Medicine, Taipei 100 Taiwan

J. Biomed. Opt. 12(1), 014014 (February 28, 2007). doi:10.1117/1.2710237
History: Received May 18, 2006; Revised August 24, 2006; Accepted August 28, 2006; Published February 28, 2007; August 21, 2009
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Intravital imaging of hepatobiliary excretion is vital for elucidating liver metabolism. In this work, we describe a novel method to observe the intravital dynamics of the uptake, processing, and excretion of an organic anion, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary system. This is achieved by the use of multiphoton microscopy and an intravital hepatic imaging chamber. The high-quality images show sequential uptake and processing of 6-CFDA from the hepatocytes and the subsequent excretion into bile canaliculi within approximately 50min. This is a promising technique to study intravital hepatic physiology and metabolism.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Yuan Liu ; Hsiao-Ching Chen ; Shu-Mei Yang ; Tzu-Lin Sun ; Wen Lo, et al.
"Visualization of hepatobiliary excretory function by intravital multiphoton microscopy", J. Biomed. Opt. 12(1), 014014 (February 28, 2007). ; http://dx.doi.org/10.1117/1.2710237


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