Research Papers

Characterization of flow direction in microchannels and zebrafish blood vessels by scanning fluorescence correlation spectroscopy

[+] Author Affiliations
Xiaotao Pan

National University of Singapore, NUS Graduate Program in Bioengineering, 28 Medical Drive, Singapore 117456 Singapore and National University of Singapore, Department of Chemistry, 3 Science Drive 3, Singapore 117543 Singapore

Hanry Yu

National University of Singapore, NUS Graduate Program in Bioengineering, 28 Medical Drive, Singapore 117456 Singapore and National University of Singapore, Department of Physiology and NUSTEP, 2 Medical Drive, Singapore 117597 Singapore and Institute of Bioengineering and Nanotechnology, A*STAR, 31 Biopolis Way, The Nanos #04-01, Singapore 138669, Singapore and Singapore-MIT Alliance, E4-04-10, 4 Engineering Drive 3, Singapore 117576 Singapore

Xianke Shi

National University of Singapore, Department of Chemistry, 3 Science Drive 3, Singapore 117543 Singapore

Vladimir Korzh

Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673 Singapore

Thorsten Wohland

National University of Singapore, NUS Graduate Program in Bioengineering, 28 Medical Drive, Singapore 117456 Singapore and National University of Singapore, Department of Chemistry, 3 Science Drive 3, Singapore 117543 Singapore

J. Biomed. Opt. 12(1), 014034 (February 02, 2007). doi:10.1117/1.2435173
History: Received May 03, 2006; Revised October 31, 2006; Accepted November 11, 2006; Published February 02, 2007
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The investigation of flow profiles in microstructures and tissues by fluorescence correlation spectroscopy (FCS) has been a challenging topic in the past decade. Due to its inherent optical configuration, a circular focused laser beam, FCS is unable to resolve microfluidic flow directions. Earlier schemes reported the use of two laser beams or the use of nonsymmetrical laser foci to break the symmetry of the measurement system. This, however, is difficult to combine with confocal systems since it would require modifications that interfere with the imaging capabilities. We propose a method called line-scan FCS to measure different flow angles in microchannels and tissues. This method is implemented on a combined laser scanning confocal microscopy (LSCM) and FCS system that enables uncompromised imaging and spectroscopy measurements. We demonstrate that by scanning the laser beam with a defined speed and direction we can measure flow direction with the current system at an optimal resolution of at least 3μm. The combination system is assessed by measuring flow profiles in a microchannel with and without obstruction. To extend the technique to live tissue measurements we demonstrate that line-scan FCS can determine the flow direction in zebrafish small blood vessels in a label-free approach.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Xiaotao Pan ; Hanry Yu ; Xianke Shi ; Vladimir Korzh and Thorsten Wohland
"Characterization of flow direction in microchannels and zebrafish blood vessels by scanning fluorescence correlation spectroscopy", J. Biomed. Opt. 12(1), 014034 (February 02, 2007). ; http://dx.doi.org/10.1117/1.2435173


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