Dependence of tissue optical properties on solute-induced changes in refractive index and osmolarity

Hanli Liu; Bertrand Beauvoit; Mika Kimura; Britton Chance

doi:10.1117/12.231370

1 April 1996 Dependence of tissue optical properties on solute-induced changes in refractive index and osmolarity

Hanli Liu, Bertrand Beauvoit, Mika Kimura, Britton Chance

Author Affiliations +

Journal of Biomedical Optics, Vol. 1, Issue 2, (April 1996). https://doi.org/10.1117/12.231370

Abstract

Additions of a solute/carbohydrate in tissue affect the size of tissue cells and the refractive indexes of the extra- and intracellular fluids, and thus the overall tissue scattering properties. We use both the Rayleigh- Gans and Mie theory approximation in calculating effects of the osmolarity and refractive indexes on the reduced scattering coefficient of tissue, and employ photon diffusion theory to associate the reduced scattering coefficient to the mean optical path length. The calculations show that changes of scattering in tissue depend not only on the change in extracellular refractive index but also on the change in osmolarity, and thus on the change in cell size and volume fraction. Experimentally, we have utilized time-domain and frequencydomain NIR techniques to measure the changes of optical properties caused by an addition of a solute in tissue models and in perfused rat livers. The temperature-dependent path length measurement of the perfused liver confirms the dependence of tissue scattering on the tissue cell size. The results obtained from the liver with three kinds of carbohydrate perfusion display different scattering aspects and can be well explained by changes in cell size and in extracellular as well as intracellular refractive indexes. The consistency between the theoretical and experimental results confirms the dependence of optical properties in (liver) tissue on both tissue osmolarity and relative refractive indexes between the extracellular and intracellular compartments. This study suggests that the NIR technique is a novel and useful tool for noninvasive, physiological monitoring.

Citation Download Citation

Hanli Liu, Bertrand Beauvoit, Mika Kimura, and Britton Chance "Dependence of tissue optical properties on solute-induced changes in refractive index and osmolarity," Journal of Biomedical Optics 1(2), (1 April 1996). https://doi.org/10.1117/12.231370

Published: 1 April 1996

Access the abstract

JOURNAL ARTICLE
12 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 179 scholarly publications and 3 patents.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Scattering

Refractive index

Liver

Tissue optics

Tissues

Mie scattering

Glucose

Show All Keywords

RELATED CONTENT

A simple analysis of extinction spectra of cancerous and normal...
Proceedings of SPIE (March 11 2015)

Measurement of optical properties of normal and pathological human liver...
Proceedings of SPIE (April 02 2020)

Mechanisms of optical clearing in cellular tissue
Proceedings of SPIE (April 15 2005)

Monte Carlo study of skin optical clearing to enhance light...
Proceedings of SPIE (June 05 2008)

Enhance light penetration in tissue for high resolution optical imaging...
Proceedings of SPIE (July 08 2003)

Simulating light propagation: towards realistic tissue models
Proceedings of SPIE (June 14 2011)

Immersion effects in tissues
Proceedings of SPIE (November 03 2000)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years