Research Papers

Blood plasma coagulation studied by surface plasmon resonance

[+] Author Affiliations
Trine P. Vikinge

IFM, Linko¨ping University, Laboratory of Applied Physics, S-581 83 Linko¨ping, Sweden

Kenny M. Hansson

Forum Scientum Graduate School, IBK, University Hospital, Division of Clinical Chemistry, S-581 85 Linko¨ping, Sweden

Johan Benesch

Forum Scientum Graduate School, IFM, Linko¨ping University, Laboratory of Applied Physics, S-581 83 Linko¨ping, Sweden

Knut Johansen

IFM, Linko¨ping University, Laboratory of Applied Physics, S-581 83 Linko¨ping, Sweden

Mats Ra˚nby, Tomas L. Lindahl

University Hospital, Department of Clinical Chemistry, S-581 85 Linko¨ping, Sweden

Bo Liedberg, Ingemar Lundsto¨m, Pentti Tengvall

IFM, Linko¨ping University, Laboratory of Applied Physics, S-581 83 Linko¨ping, Sweden

J. Biomed. Opt. 5(1), 51-55 (Jan 01, 2000). doi:10.1117/1.429968
History: Received Dec. 16, 1998; Revised Oct. 19, 1999; Accepted Oct. 26, 1999
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Abstract

A surface plasmon resonance (SPR) apparatus was used to investigate blood plasma coagulation in real time as a function of thromboplastin and heparin concentrations. The response curves were analyzed by curve fitting to a sigmoid curve equation, followed by extraction of the time constant. Clotting activation by thromboplastin resulted in increased time constant, as compared to spontaneously clotted plasma, in a dose dependent way. Addition of heparin to the thromboplastin-activated plasma counteracted this effect. Atomic force microscopy (AFM) pictures of sensor surfaces dried after completed clotting, revealed differences in fibrin network structures as a function of thromboplastin concentration, and the fiber thickness increased with decreased thromboplastin concentration. The physical reason for the SPR signal observed is ambiguous and is therefore discussed. However, the results summarized in the plots and the fibrin network properties observed by AFM correlate well with present common methods used to analyze blood coagulation. © 2000 Society of Photo-Optical Instrumentation Engineers.

© 2000 Society of Photo-Optical Instrumentation Engineers

Citation

Trine P. Vikinge ; Kenny M. Hansson ; Johan Benesch ; Knut Johansen ; Mats Ra˚nby, et al.
"Blood plasma coagulation studied by surface plasmon resonance", J. Biomed. Opt. 5(1), 51-55 (Jan 01, 2000). ; http://dx.doi.org/10.1117/1.429968


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