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SPECIAL SECTION ON FRONTIERS IN MICROSCOPY

Second-harmonic imaging microscopy of living cells

[+] Author Affiliations
Paul J. Campagnola, Heather A. Clark, William A. Mohler

Center for Biomedical Imaging Technology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut?06030

Aaron Lewis

Division of Applied Optics, Hebrew University, Jerusalem Israel

Leslie M. Loew

Center for Biomedical Imaging Technology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut?06030

J. Biomed. Opt. 6(3), 277-286 (Jul 01, 2001). doi:10.1117/1.1383294
History: Received Apr. 2, 2001; Accepted Apr. 20, 2001
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Second harmonic generation (SHG) has been developed in our laboratories as a high-resolution nonlinear optical imaging microscopy for cellular membranes and intact tissues. SHG shares many of the advantageous features for microscopy of another more established nonlinear optical technique: two-photon excited fluorescence (TPEF). Both are capable of optical sectioning to produce three-dimensional images of thick specimens and both result in less photodamage to living tissue than confocal microscopy. SHG is complementary to TPEF in that it uses a different contrast mechanism and is most easily detected in the transmitted light optical path. It can be used to image membrane probes with high membrane specificity and displays extraordinary sensitivity in reporting membrane potential; it also has the ability to image highly ordered structural proteins without any exogenous labels. © 2001 Society of Photo-Optical Instrumentation Engineers.

Figures in this Article
© 2001 Society of Photo-Optical Instrumentation Engineers

Citation

Paul J. Campagnola ; Aaron Lewis ; Leslie M. Loew ; Heather A. Clark and William A. Mohler
"Second-harmonic imaging microscopy of living cells", J. Biomed. Opt. 6(3), 277-286 (Jul 01, 2001). ; http://dx.doi.org/10.1117/1.1383294


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