0
SPECIAL SECTION ON TISSUE POLARIMETRY

Polarized angular dependent spectroscopy of epithelial cells and epithelial cell nuclei to determine the size scale of scattering structures

[+] Author Affiliations
J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, J. P. Freyer

Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico?87545

J. Biomed. Opt. 7(3), 378-387 (Jul 01, 2002). doi:10.1117/1.1483317
History: Received Sep. 27, 2001; Revised Feb. 7, 2002; Accepted Mar. 11, 2002; Online August 01, 2002
Text Size: A A A

An understanding of the relationship between tissue structures and light scattering from tissue will help facilitate the development and acceptance of noninvasive optical diagnostics including elastic scattering spectroscopy, diffuse reflectance, and optical coherence tomography. For example, a quantitative model of the structures that scatter light in epithelial cells would allow determination of what structures control the characteristics of in vivo light transport measurements and subsequently could provide a detailed relationship between cellular structures and optical measurements. We have determined the size distribution of refractive index structure variations in epithelial cells as well as in nuclei isolated from epithelial cells from measurements of the angular dependence of polarized light scattering. The quantitative size distributions we obtained for both whole cells and isolated nuclei include particles with effective radii of 2 μm to 10 nm or less and contain orders of magnitude more small particles than large particles. These results demonstrate that not only are biological cells very heterogeneous, but so are the nuclei within them. Light scattering is likely sensitive to structures smaller than those commonly investigated by standard pathology methods. © 2002 Society of Photo-Optical Instrumentation Engineers.

© 2002 Society of Photo-Optical Instrumentation Engineers

Citation

J. R. Mourant ; T. M. Johnson ; S. Carpenter ; A. Guerra ; T. Aida, et al.
"Polarized angular dependent spectroscopy of epithelial cells and epithelial cell nuclei to determine the size scale of scattering structures", J. Biomed. Opt. 7(3), 378-387 (Jul 01, 2002). ; http://dx.doi.org/10.1117/1.1483317


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In