Research Papers

Contrast agents for confocal microscopy: how simple chemicals affect confocal images of normal and cancer cells in suspension

[+] Author Affiliations
Andre´s F. Zuluaga, Rebekah Drezek

The University of Texas at Austin, Department of Electrical and Computer Engineering, Austin, Texas

Tom Collier

The University of Texas at Austin, The Biomedical Engineering Program, Austin, Texas

Reuben Lotan

The University of Texas M.D. Anderson Cancer Center, Department of Tumor Biology, Houston, Texas

Michele Follen

The University of Texas M.D. Anderson Cancer Center, Department of Gynecologic Oncology, Houston, Texas and The University of Texas Health Science Center at Houston, Department of Obstetrics, Gynecology and Reproductive Sciences, Houston, Texas

Rebecca Richards-Kortum

The University of Texas at Austin, The Biomedical Engineering Program, Austin, Texas

J. Biomed. Opt. 7(3), 398-403 (Jul 01, 2002). doi:10.1117/1.1481047
History: Received Nov. 13, 2000; Revised Apr. 30, 2001; Accepted Apr. 30, 2001; Online August 01, 2002
Text Size: A A A

Normal and malignant human cervical cancer cells were imaged in vivo with confocal, phase contrast, and brightfield microscopies. Results were compared between cells in growth medium before and after addition of acetic acid, hypertonic saline solution, toluidine blue, and Lugol’s iodine. The exogenous agents changed the backscattering characteristics of the cells when measured with confocal microscopy at 808 nm. A tendency toward higher scattering was observed in treated cells. Acetic acid and toluidine blue increased the brightness of the nucleus with respect to the cytoplasm in normal and cancer cells. Hypertonic saline solution made the cytoplasm brighter than the nucleus in both types of cells. The results indicate that simple chemicals can be used to enhance confocal microscopy’s ability to differentiate intracellular components, such as nuclear size and shape. This can further confocal microscopy’s ability to assess disease in cells and tissues. © 2002 Society of Photo-Optical Instrumentation Engineers.

© 2002 Society of Photo-Optical Instrumentation Engineers

Citation

Andre´s F. Zuluaga ; Rebekah Drezek ; Tom Collier ; Reuben Lotan ; Michele Follen, et al.
"Contrast agents for confocal microscopy: how simple chemicals affect confocal images of normal and cancer cells in suspension", J. Biomed. Opt. 7(3), 398-403 (Jul 01, 2002). ; http://dx.doi.org/10.1117/1.1481047


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.