Research Papers

Fluorescence spectroscopy for cervical precancer detection: Is there variance across the menstrual cycle?

[+] Author Affiliations
Sung K. Chang

University of Texas at Austin, Department of Biomedical Engineering, Austin, Texas?78712

M. Yusoff Dawood

The University of Texas Health Sciences Center, Department of Obstetrics, Gynecology and Reproductive Sciences, Houston, Texas?77030

Gregg Staerkel

University of Texas M.D. Anderson Cancer Center, Department of Anatomic Pathology, Houston, Texas?77030

Urs Utzinger

The University of Arizona, Department of Biomedical Engineering, Tucson, Arizona?85721

E. Neely Atkinson

University of Texas M.D. Anderson Cancer Center, Department of Biomathematics, Houston, Texas?77030

Rebecca R. Richards-Kortum

University of Texas at Austin, Department of Biomedical Engineering, Engineering Science Building, Room 8, Austin, Texas?78712

Michele Follen

The University of Texas Health Sciences Center, Department of Obstetrics, Gynecology and Reproductive Sciences, Houston, Texas?77030

and University of Texas M.D. Anderson Cancer Center, Department of Gynecologic Oncology, Houston, Texas?77030

J. Biomed. Opt. 7(4), 595-602 (Oct 01, 2002). doi:10.1117/1.1509753
History: Received Oct. 18, 2001; Revised May 29, 2002; Accepted June 12, 2002; Online October 22, 2002
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This study assesses one possible cause of inter-patient variation in fluorescence spectroscopy of the cervix: the menstrual cycle. Ten patients with no history of an abnormal Pap smear were seen daily throughout 30 consecutive days of their cycle. Fluorescence excitation-emission matrices were measured from three cervical sites on each patient. Principal component analysis was used to determine which spectral regions varied with the day of the cycle. Classification was performed to assess the influence of menstrual cycle on precancer diagnosis. Variations in the principal component scores and the redox ratio values show that the fluorescence emission spectra at 340–380 nm excitation appear to correlate with the cell metabolism of the cervical epithelium throughout the menstrual cycle; these changes do not affect diagnostic classification. The menstrual cycle affects intra-patient variation but does not appear to cause a significant level of inter-patient variation. It does not need to be controlled for in optical detection strategies based on fluorescence spectroscopy. © 2002 Society of Photo-Optical Instrumentation Engineers.

© 2002 Society of Photo-Optical Instrumentation Engineers

Citation

Sung K. Chang ; M. Yusoff Dawood ; Gregg Staerkel ; Urs Utzinger ; E. Neely Atkinson, et al.
"Fluorescence spectroscopy for cervical precancer detection: Is there variance across the menstrual cycle?", J. Biomed. Opt. 7(4), 595-602 (Oct 01, 2002). ; http://dx.doi.org/10.1117/1.1509753


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