Research Papers

Spatial localization of cardiac optical mapping with multiphoton excitation

[+] Author Affiliations
Venkat K. Ramshesh, Stephen B. Knisley

The University of North Carolina at Chapel Hill, The Department of Biomedical Engineering of the School of Medicine, CB #7575, 152 MacNider Hall, Chapel Hill, North Carolina 27599-7575 E-mail: knisley@bme.unc.edu

J. Biomed. Opt. 8(2), 253-259 (Apr 01, 2003). doi:10.1117/1.1559831
History: Received Apr. 26, 2002; Revised Oct. 30, 2002; Accepted Nov. 18, 2002; Online April 01, 2003
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Depth and radius of regions interrogated by cardiac optical mapping with a laser beam depend on photon travel inside the heart. It would be useful to limit the range of depth and radius interrogated. We modeled the effects of a condensing lens to concentrate laser light at a target depth inside the heart, and near infrared excitation to increase penetration and produce two-photon absorption. A Monte Carlo simulation that incorporated a 0.55-NA lens, and absorption and scattering of 1064- or 488-nm laser light in 3-D cardiac tissue indicated the distribution of excitation fluence inside the tissue. A subsequent simulation incorporating absorption and scattering of transmembrane voltage-sensitive fluorescence (wavelength 669 nm) indicated locations from which fluorescence photons exiting the tissue surface originated. The results indicate that mapping at depths up to 300 μm in hearts can provide significant improvement in localization over existing cardiac optical mapping. The estimated interrogation region is sufficiently small to examine cardiac events at a cellular or subcellular scale and may allow mapping at various depths in the heart. © 2003 Society of Photo-Optical Instrumentation Engineers.

© 2003 Society of Photo-Optical Instrumentation Engineers

Citation

Venkat K. Ramshesh and Stephen B. Knisley
"Spatial localization of cardiac optical mapping with multiphoton excitation", J. Biomed. Opt. 8(2), 253-259 (Apr 01, 2003). ; http://dx.doi.org/10.1117/1.1559831


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