SPECIAL SECTION ON MULTIPHOTON MICROSCOPY

Quantitative imaging of protein–protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera

[+] Author Affiliations
R. V. Krishnan, A. Masuda, V. E. Centonze, B. Herman

University of Texas Health Science Center, Department of Cellular and Structural Biology, 7703 Floyd Curl Drive, San Antonio, Texas?78229

J. Biomed. Opt. 8(3), 362-367 (Jul 01, 2003). doi:10.1117/1.1577574
History: Received Dec. 9, 2002; Revised Mar. 10, 2003; Accepted Mar. 10, 2003; Online July 18, 2003
Text Size: A A A

Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein–protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured. © 2003 Society of Photo-Optical Instrumentation Engineers.

© 2003 Society of Photo-Optical Instrumentation Engineers

Citation

R. V. Krishnan ; A. Masuda ; V. E. Centonze and B. Herman
"Quantitative imaging of protein–protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera", J. Biomed. Opt. 8(3), 362-367 (Jul 01, 2003). ; http://dx.doi.org/10.1117/1.1577574


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.