SPECIAL SECTION ON MULTIPHOTON MICROSCOPY

Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods

[+] Author Affiliations
Enrico Gratton, Sophie Breusegem, Jason Sutin, Qiaoqiao Ruan, Nicholas Barry

University of Illinois at Urbana-Champaign, Laboratory for Fluorescence Dynamics, 1110 West Green Street Urbana, Illinois?61801 E-mail: Enrico@scs.uiuc.edu

J. Biomed. Opt. 8(3), 381-390 (Jul 01, 2003). doi:10.1117/1.1586704
History: Received Apr. 8, 2003; Revised Apr. 10, 2003; Accepted Apr. 10, 2003
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Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples. © 2003 Society of Photo-Optical Instrumentation Engineers.

© 2003 Society of Photo-Optical Instrumentation Engineers

Citation

Enrico Gratton ; Sophie Breusegem ; Jason Sutin ; Qiaoqiao Ruan and Nicholas Barry
"Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods", J. Biomed. Opt. 8(3), 381-390 (Jul 01, 2003). ; http://dx.doi.org/10.1117/1.1586704


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