0
SPECIAL SECTION ON MULTIPHOTON MICROSCOPY

Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium

[+] Author Affiliations
Chen-Yuan Dong

National Taiwan University, Microscopic Biophysics Laboratory, Department of Physics, Taipei?106, Taiwan

Karsten Koenig

Friedrich Schiller University Jena, Institute of Anatomy II, Teichgraben 7, D-07743?Jena, Germany

Peter So

Massachusetts Institute of Technology, Department of Mechanical Engineering, 77 Massachusetts Ave., Cambridge, Massachusetts?02139, USA E-mail: ptso@mit.edu

J. Biomed. Opt. 8(3), 450-459 (Jul 01, 2003). doi:10.1117/1.1578644
History: Received Sep. 3, 2002; Revised Mar. 14, 2003; Accepted Mar. 17, 2003; Online July 18, 2003
Text Size: A A A

In recent years, fluorescence microscopy based on two-photon excitation has become a popular tool for biological and biomedical imaging. Among its advantages is the enhanced depth penetration permitted by fluorescence excitation with the near-infrared photons, which is particularly attractive for deep-tissue imaging. To fully utilize two-photon fluorescence microscopy as a three-dimensional research technique in biology and medicine, it is important to characterize the two-photon imaging parameters in a turbid medium. We investigated the two-photon point spread functions (PSFs) in a number of scattering samples. Gel samples containing 0.1-μm fluorescent microspheres and Liposyn III were used as phantoms mimicking the turbid environment often found in tissue. A full characterization of the two-photon PSFs of a water and oil immersion objective was completed in samples composed of 0, 0.25, 0.5, 1, and 2% Liposyn III. Our results show that up to depths of about 100 (oil) and 200 μm (water), the presence of scatterers (up to 2% Liposyn III) does not appreciably degrade the PSF widths of the objectives. © 2003 Society of Photo-Optical Instrumentation Engineers.

© 2003 Society of Photo-Optical Instrumentation Engineers

Citation

Chen-Yuan Dong ; Karsten Koenig and Peter So
"Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium", J. Biomed. Opt. 8(3), 450-459 (Jul 01, 2003). ; http://dx.doi.org/10.1117/1.1578644


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In