Research Papers

In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties

[+] Author Affiliations
Philippe Thueler

Swiss Federal Institute of Technology, Applied Optics Institute, PO Box 127, CH-1015 Lausanne, Switzerland E-mail: philippe.thueler@bigfoot.com

Igor Charvet

University of Geneva, School of Medicine, Department of Morphology, Rue Michel Servet 1, CH-1211 Geneva 4, Switzerland

Frederic Bevilacqua

Swiss Federal Institute of Technology, Applied Optics Institute, PO Box 127, CH-1015 Lausanne, Switzerland and University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, 1002 Health Sciences Road East, Irvine, California 92612

M. St. Ghislain

Swiss Federal Institute of Technology, Applied Optics Institute, PO Box 127, CH-1015 Lausanne, Switzerland

G. Ory

Geneva University Hospital, Internal Medicine Department, Rue Micheli-du-Crest 24, CH-1205 Geneva, Switzerland

Pierre Marquet

University of Lausanne, Institute of Physiology, R. Bugnon 7, CH-1005 Lausanne, Switzerland

Paolo Meda

University of Geneva, School of Medicine, Department of Morphology, Rue Michel Servet 1, CH-1211 Geneva 4, Switzerland

Ben Vermeulen

Geneva University Hospital, Internal Medicine Department, Rue Micheli-du-Crest 24, CH-1205 Geneva, Switzerland

Christian Depeursinge

Swiss Federal Institute of Technology, Applied Optics Institute, PO Box 127, CH-1015 Lausanne, Switzerland

J. Biomed. Opt. 8(3), 495-503 (Jul 01, 2003). doi:10.1117/1.1578494
History: Received Aug. 7, 2002; Revised Dec. 16, 2002; Accepted Jan. 7, 2003; Online July 18, 2003
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A fast spectroscopic system for superficial and local determination of the absorption and scattering properties of tissue (480 to 950 nm) is described. The probe can be used in the working channel of an endoscope. The scattering properties include the reduced scattering coefficient and a parameter of the phase function called γ, which depends on its first two moments. The inverse problem algorithm is based on the fit of absolute reflectance measurements to cubic B-spline functions derived from the interpolation of a set of Monte Carlo simulations. The algorithm’s robustness was tested with simulations altered with various amounts of noise. The method was also assessed on tissue phantoms of known optical properties. Finally, clinical measurements performed endoscopically in vivo in the stomach of human subjects are presented. The absorption and scattering properties were found to be significantly different in the antrum and in the fundus and are correlated with histopathologic observations. The method and the instrument show promise for noninvasive tissue diagnostics of various epithelia. © 2003 Society of Photo-Optical Instrumentation Engineers.

© 2003 Society of Photo-Optical Instrumentation Engineers

Citation

Philippe Thueler ; Igor Charvet ; Frederic Bevilacqua ; M. St. Ghislain ; G. Ory, et al.
"In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties", J. Biomed. Opt. 8(3), 495-503 (Jul 01, 2003). ; http://dx.doi.org/10.1117/1.1578494


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