Research Papers

Assessment of expressions of heat shock protein (HSP 72) and apoptosis after ArF excimer laser ablation of the cornea

[+] Author Affiliations
Miya Ishihara, Masato Sato

National Defense Medical College, Department of Medical Engineering, Saitama, Japan E-mail: kobako@cc.ndmc.ac.jp

Shunichi Sato

National Defense Medical College Research Institute, Division of Biomedical Information Sciences, Saitama, Japan

Tsunenori Arai

Keio University, Faculty of Science and Technology, Department of Applied Physics, and Physico-Informatics, Yokohama, Japan

Minoru Obara

Keio University, Faculty of Science and Technology, Department of Electrical Engineering, Yokohama, Japan

Makoto Kikuchi

National Defense Medical College, Department of Medical Engineering, Saitama, Japan

J. Biomed. Opt. 9(1), 187-192 (Jan 01, 2004). doi:10.1117/1.1630605
History: Received Dec. 29, 2002; Revised Jun. 30, 2003; Accepted Jul. 1, 2003; Online January 07, 2004
Text Size: A A A

We immunohistochemically studied expressions of inducible heat shock protein 70 (HSP 72) and apoptosis of corneas ablated with an ArF excimer laser. The temperature of corneal surfaces and laser-induced optical emission spectra were measured in real time as direct physical parameters related to the ablation mechanism. To the best of the authors’ knowledge, there have been no experimental studies regarding the influence of physical parameters directly related to the ablation mechanism on corneal reactions at the cell level after laser ablation. The expression of HSP 72 was mainly localized in the regenerative epithelium, which was confirmed to be caused by laser ablation. The HSP 72 positive cell ratios had a correlation with thermal dose, which was derived from the measured time courses of temperature. Expressions of both HSP 72 and apoptosis depended on the thermal dose and elapsed time after ablation. HSP 72 and apoptosis could be seen up to a few hundred micrometers into the stroma, only at a fluence with an optical breakdown emission. This could have been caused by shock waves induced by the optical breakdown. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Miya Ishihara ; Masato Sato ; Shunichi Sato ; Tsunenori Arai ; Minoru Obara, et al.
"Assessment of expressions of heat shock protein (HSP 72) and apoptosis after ArF excimer laser ablation of the cornea", J. Biomed. Opt. 9(1), 187-192 (Jan 01, 2004). ; http://dx.doi.org/10.1117/1.1630605


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.