Special Section on Optics of Human Skin

Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry

[+] Author Affiliations
Takeshi Yasui

Osaka University, Graduate School of Engineering Science, Toyonaka, Osaka, 560-8531 Japan

Yoshiyuki Tohno

Nara Medical University, Department of 1st Anatomy Kashihara, Nara 634-8521, Japan

Tsutomu Araki

Osaka University, Graduate School of Engineering Science, Toyonaka, Osaka, 560-8531 Japan

J. Biomed. Opt. 9(2), 259-264 (Mar 01, 2004). doi:10.1117/1.1644116
History: Received Mar. 27, 2003; Revised Aug. 19, 2003; Revised Nov. 14, 2003; Accepted Nov. 17, 2003; Online March 22, 2004
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We have proposed an optical probe that can be used to characterize the orientation of collagen fibers in human dermis. A specific probing ability for collagen results from the use of second-harmonic-generation (SHG) light induced by collagen molecules in the tissue. Based on the concept of collagen SHG light, a reflection-type polarization measurement system (named SHG polarimetry) with a probe light spot of 15 μm in diameter has been constructed, and the human reticular dermis has been measured using this system. Resultant data exhibit that the reticular dermis possesses approximately uniaxial orientation of the collagen fibers. Furthermore, we demonstrated a nondestructive measurement of the collagen orientation in the papillary dermis across an epidermis layer. For distribution measurement of the collagen fiber orientation in the reticular dermis, we have extended the SHG polarimetry to one- (1-D) and two-dimensional (2-D) measurement. By the macroscopic 2-D SHG polarimetry, we have observed that the orientation angle and organization degree of collagen fibers vary widely depending on the discrete probing positions in the reticular dermis. Furthermore, microscopic 1-D SHG polarimetry indicated a swell of the orientation angle and a large variance of the organization degree in the collagen fibers in the microscopic region. These results imply that the reticular dermis posses a tangled structure of collagen fibers, which is highly consistent with the result of the anatomical examination of the skin. The proposed method will be a powerful tool for monitoring the microscopic distribution of the collagen fiber orientation in the human dermis. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Takeshi Yasui ; Yoshiyuki Tohno and Tsutomu Araki
"Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry", J. Biomed. Opt. 9(2), 259-264 (Mar 01, 2004). ; http://dx.doi.org/10.1117/1.1644116


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