Special Section on Optics of Human Skin

Amending of fluorescence sensor signal localization in human skin by matching of the refractive index

[+] Author Affiliations
Dmitry Y. Churmakov, Igor V. Meglinski, Douglas A. Greenhalgh

Cranfield University, School of Engineering, Cranfield MK43 0AL, United Kingdom

J. Biomed. Opt. 9(2), 339-346 (Mar 01, 2004). doi:10.1117/1.1645796
History: Received Apr. 8, 2003; Revised Jun. 18, 2003; Accepted Jun. 19, 2003; Online March 22, 2004
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Fluorescence diagnostic techniques are notable amongst many other optical methods because they offer high sensitivity and noninvasive measurement of tissue properties. However, a combination of multiple scattering and physical heterogeneity of biological tissues hampers interpretation of the fluorescence measurements. Analyses of the spatial distribution of endogenous and exogenous fluorophores excitation within tissues and their contribution to the detected signal localization are essential for many applications. We have developed a novel Monte Carlo technique that gives a graphical perception of how the excitation and fluorescence detected signal are localized in tissues. Our model takes into account the spatial distribution of fluorophores, the variation of concentrations and quantum yield. We demonstrate that matching the refractive indices of the ambient medium and topical skin layer improves spatial localization of the detected fluorescence signal within the tissues. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Dmitry Y. Churmakov ; Igor V. Meglinski and Douglas A. Greenhalgh
"Amending of fluorescence sensor signal localization in human skin by matching of the refractive index", J. Biomed. Opt. 9(2), 339-346 (Mar 01, 2004). ; http://dx.doi.org/10.1117/1.1645796


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