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Special Section on Biomedical Optics and Women's Health

Diagnostic imaging of breast cancer using fluorescence-enhanced optical tomography: phantom studies

[+] Author Affiliations
A. Godavarty, A. B. Thompson, R. Roy, M. Gurfinkel

Texas A&M University, Photon Migration Laboratory, College Station, Texas?77843-3573

M. J. Eppstein, C. Zhang

University of Vermont, Department of Computer Science, Burlington, Vermont?05405

E. M. Sevick-Muraca

Texas A&M University, Photon Migration Laboratory, College Station, Texas?77843-3573

E-mail: eva-m-sevick@tamu.edu

J. Biomed. Opt. 9(3), 488-496 (May 01, 2004). doi:10.1117/1.1691027
History: Received Jul. 7, 2003; Revised Dec. 10, 2003; Accepted Dec. 19, 2003; Online April 21, 2004
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Molecular targeting with exogenous near-infrared excitable fluorescent agents using time-dependent imaging techniques may enable diagnostic imaging of breast cancer and prognostic imaging of sentinel lymph nodes within the breast. However, prior to the administration of unproven contrast agents, phantom studies on clinically relevant volumes are essential to assess the benefits of fluorescence-enhanced optical imaging in humans. Diagnostic 3-D fluorescence-enhanced optical tomography is demonstrated using 0.5 to 1cm3 single and multiple targets differentiated from their surroundings by indocyanine green (micromolar) in a breast-shaped phantom (10-cm diameter). Fluorescence measurements of referenced ac intensity and phase shift were acquired in response to point illumination measurement geometry using a homodyned intensified charge-coupled device system modulated at 100 MHz. Bayesian reconstructions show artifact-free 3-D images (3857 unknowns) from 3-D boundary surface measurements (126 to 439). In a reflectance geometry appropriate for prognostic imaging of lymph node involvement, fluorescence measurements were likewise acquired from the surface of a semi-infinite phantom (8×8×8cm3) in response to area illumination (12cm2) by excitation light. Tomographic 3-D reconstructions (24,123 unknowns) were recovered from 2-D boundary surface measurements (3194) using the modified truncated Newton’s method. These studies represent the first 3-D tomographic images from physiologically relevant geometries for breast imaging. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

A. Godavarty ; A. B. Thompson ; R. Roy ; M. Gurfinkel ; M. J. Eppstein, et al.
"Diagnostic imaging of breast cancer using fluorescence-enhanced optical tomography: phantom studies", J. Biomed. Opt. 9(3), 488-496 (May 01, 2004). ; http://dx.doi.org/10.1117/1.1691027


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