Special Section on Biomedical Optics and Women's Health

Analytical model to describe fluorescence spectra of normal and preneoplastic epithelial tissue: comparison with Monte Carlo simulations and clinical measurements

[+] Author Affiliations
Sung K. Chang, Dizem Arifler

University of Texas at Austin, Department of Biomedical Engineering, Austin, Texas?78712

Rebekah Drezek

Rice University, Department of Bioengineering, Houston, Texas?77005

Michele Follen

University of Texas, M.D. Anderson Cancer Center, Department of Gynecologic Oncology, and Center for Biomedical Engineering, Houston, Texas?77030

Rebecca Richards-Kortum

University of Texas at Austin, Department of Biomedical Engineering, Austin, Texas?78712 E-mail: kortum@mail.utexas.edu

J. Biomed. Opt. 9(3), 511-522 (May 01, 2004). doi:10.1117/1.1695559
History: Received Aug. 7, 2003; Revised Dec. 12, 2003; Accepted Dec. 19, 2003; Online April 21, 2004
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Fluorescence spectroscopy has shown promise for the detection of precancerous changes in vivo. The epithelial and stromal layers of tissue have very different optical properties; the albedo is relatively low in the epithelium and approaches one in the stroma. As precancer develops, the optical properties of the epithelium and stroma are altered in markedly different ways: epithelial scattering and fluorescence increase, and stromal scattering and fluorescence decrease. We present an analytical model of the fluorescence spectrum of a two-layer medium such as epithelial tissue. Our hypothesis is that accounting for the two different tissue layers will provide increased diagnostic information when used to analyze tissue fluorescence spectra measured in vivo. The Beer-Lambert law is used to describe light propagation in the epithelial layer, while light propagation in the highly scattering stromal layer is described with diffusion theory. Predictions of the analytical model are compared to results from Monte Carlo simulations of light propagation under a range of optical properties reported for normal and precancerous epithelial tissue. In all cases, the mean square error between the Monte Carlo simulations and the analytical model are within 15%. Finally, model predictions are compared to fluorescence spectra of normal and precancerous cervical tissue measured in vivo; the lineshape of fluorescence agrees well in both cases, and the decrease in fluorescence intensity from normal to precancerous tissue is correctly predicted to within 5%. Future work will explore the use of this model to extract information about changes in epithelial and stromal optical properties from clinical measurements and the diagnostic value of these parameters. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Sung K. Chang ; Dizem Arifler ; Rebekah Drezek ; Michele Follen and Rebecca Richards-Kortum
"Analytical model to describe fluorescence spectra of normal and preneoplastic epithelial tissue: comparison with Monte Carlo simulations and clinical measurements", J. Biomed. Opt. 9(3), 511-522 (May 01, 2004). ; http://dx.doi.org/10.1117/1.1695559


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