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Special Section on Biomedical Optics and Women's Health

Characterization of hemoglobin, water, and NIR scattering in breast tissue: analysis of intersubject variability and menstrual cycle changes

[+] Author Affiliations
Brian W. Pogue

Dartmouth College, Thayer School of Engineering, 800 Cummings Hall, Hanover, New Hampshire 03755

Dartmouth Hitchcock Medical Center, Norris Cotton Cancer Center, Lebanon, New Hampshire 03756 E-mail: pogue@dartmouth.edu

Shudong Jiang, Hamid Dehghani

Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire 03755

Christine Kogel, Sandra Soho

Dartmouth Hitchcock Medical Center, Norris Cotton Cancer Center, Lebanon, New Hampshire 03756

Subhadra Srinivasan, Xiaomei Song

Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire 03755

Tor D. Tosteson

Dartmouth Hitchcock Medical Center, Norris Cotton Cancer Center, Lebanon, New Hampshire 03756

Steven P. Poplack

Dartmouth Hitchcock Medical Center, Norris Cotton Cancer Center, Lebanon, New Hampshire 03756

Dartmouth Medical School, Department of Radiology, Hanover, New Hampshire 03755

Keith D. Paulsen

Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, New Hampshire 03755

Dartmouth Hitchcock Medical Center, Norris Cotton Cancer Center, Lebanon, New Hampshire 03756

J. Biomed. Opt. 9(3), 541-552 (May 01, 2004). doi:10.1117/1.1691028
History: Received Jul. 15, 2003; Revised Aug. 21, 2003; Accepted Sep. 4, 2003; Online April 21, 2004
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Near-infrared imaging was used to quantify typical values of hemoglobin concentration, oxygen saturation, water fraction, scattering power, and scattering amplitude within the breast tissue of volunteer subjects. A systematic study of the menstrual variations in these parameters was carried out by measuring a group of seven premenopausal normal women (aged 41 to 47 years) in the follicular (days 7 to 14 of the cycle) and secretory phases (days 21 to 28) of the cycle, for two complete menstrual cycles. An average increase in hemoglobin concentration of 2.6 μM or 13% of the background breast values was observed in the secretory phase relative to the follicular phase (p<0.0001), but no other average near-infrared parameter changes were significant. While repeatable and systematic changes were observed in all parameters for individual subjects, large intersubject variations were present in all parameters. In a survey of thirty-nine normal subjects, the total hemoglobin varied from 9 to 45 μM, with a systematic correlation observed between total hemoglobin concentration and breast radiographic density. Scattering power and scattering amplitude were also correlated with radiographic density, but oxygen saturation and water fraction were not. Images of breast lesions indicate that total hemoglobin-based contrast can be up to 200% relative to the background in the same breast. Yet, since the background hemoglobin values vary considerably among breasts, the maximum hemoglobin concentrations observed in cancer tumors may vary considerably as well. In light of these observations, it may be important to use hemoglobin contrast values relative to the background for a given breast, rather than absolute hemoglobin contrast when trying to compare the features of breast lesions among subjects. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Brian W. Pogue ; Shudong Jiang ; Hamid Dehghani ; Christine Kogel ; Sandra Soho, et al.
"Characterization of hemoglobin, water, and NIR scattering in breast tissue: analysis of intersubject variability and menstrual cycle changes", J. Biomed. Opt. 9(3), 541-552 (May 01, 2004). ; http://dx.doi.org/10.1117/1.1691028


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