Research Papers

Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice

[+] Author Affiliations
S. Bhaumik, X. Z. Lewis

The Crump Institute for Molecular Imaging, 700 Westwood Boulevard, Los Angeles, California?90095-1700

S. S. Gambhir

Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, The James H. Clark Center, 318 Campus Drive, East Wing, First Floor, E150A, Stanford, California 94305-5427 E-mail: sgambhir@stanford.edu

J. Biomed. Opt. 9(3), 578-586 (May 01, 2004). doi:10.1117/1.1647546
History: Received Feb. 28, 2003; Revised Jul. 23, 2003; Accepted Aug. 1, 2003; Online April 21, 2004
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We have recently demonstrated that Renilla luciferase (Rluc) is a promising bioluminescence reporter gene that can be used for noninvasive optical imaging of reporter gene expression in living mice, with the aid of a cooled charged couple device (CCD) camera. In the current study, we explore the expression of a novel synthetic Renilla luciferase reporter gene (hRluc) in living mice, which has previously been reported to be a more sensitive reporter than native Rluc in mammalian cells. We explore the strategies of simultaneous imaging of both Renilla luciferase enzyme (RL) and synthetic Renilla luciferase enzyme (hRL):coelenterazine (substrate for RL/hRL) in the same living mouse. We also demonstrate that hRL:coelenterazine can yield a higher signal when compared to Firefly luciferase enzyme (FL): D-Luciferin, both in cell culture studies and when imaged from cells at the surface and from lungs of living mice. These studies demonstrate that hRluc should be a useful primary reporter gene with high sensitivity when used alone or in conjunction with other bioluminescence reporter genes for imaging in living rodents. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

S. Bhaumik ; X. Z. Lewis and S. S. Gambhir
"Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice", J. Biomed. Opt. 9(3), 578-586 (May 01, 2004). ; http://dx.doi.org/10.1117/1.1647546


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