Research Papers

Resonance Raman spectroscopy of optically trapped functional erythrocytes

[+] Author Affiliations
Kerstin Ramser

Chalmers University of Technology, Go¨teborg University, Department of Experimental Physics, S-412?96?Go¨teborg, Sweden E-mail: k.ramser@fy.chalmers.se

Katarina Logg

Chalmers University of Technology, Department of Applied Physics, S-412?96?Go¨teborg, Sweden

Mattias Gokso¨r, Jonas Enger

Chalmers University of Technology, Go¨teborg University, Department of Experimental Physics, S-412?96?Go¨teborg, Sweden

Mikael Ka¨ll

Chalmers University of Technology, Department of Applied Physics, S-412?96?Go¨teborg, Sweden

Dag Hanstorp

Chalmers University of Technology, Go¨teborg University, Department of Experimental Physics, S-412?96?Go¨teborg, Sweden

J. Biomed. Opt. 9(3), 593-600 (May 01, 2004). doi:10.1117/1.1689336
History: Received Mar. 6, 2003; Revised Aug. 20, 2003; Accepted Aug. 21, 2003; Online April 21, 2004
Text Size: A A A

We introduce a novel setup combining a micro-Raman spectrometer with external optical tweezers, suitable for resonance Raman studies of single functional trapped cells. The system differs from earlier setups in that two separate laser beams used for trapping and Raman excitation are combined in a double-microscope configuration. This has the advantage that the wavelength and power of the trapping and probe beam can be adjusted individually to optimize the functionality of the setup and to enable the recording of resonance Raman profiles from a single trapped cell. Trapping is achieved by tightly focusing infrared (IR) diode laser radiation (830 nm) through an inverted oil-immersion objective, and resonance Raman scattering is excited by the lines of an argon:krypton ion laser. The functionality of the system is demonstrated by measurements of trapped single functional erythrocytes using different excitation lines (488.0, 514.5, and 568.2 nm) in resonance with the heme moiety and by studying spectral evolution during illumination. We found that great care has to be taken in order to avoid photodamage caused by the visible Raman excitation, whereas the IR trapping irradiation does not seem to harm the cells or alter the hemoglobin Raman spectra. Stronger photodamage is induced by Raman excitation using 488.0- and 514.5-nm irradiation, compared with excitation with the 568.2-nm line. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Kerstin Ramser ; Katarina Logg ; Mattias Gokso¨r ; Jonas Enger ; Mikael Ka¨ll, et al.
"Resonance Raman spectroscopy of optically trapped functional erythrocytes", J. Biomed. Opt. 9(3), 593-600 (May 01, 2004). ; http://dx.doi.org/10.1117/1.1689336


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Raman spectrometers and microscopes. J AOAC Int ;88(2):37A-44A.
Advertisement

 

 

 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.