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Research Papers

Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation

[+] Author Affiliations
Guenter Giese

Max-Planck-Institute for Medical Research, Jahnstrasse 29, D-69120?Heidelberg, Germany

Matthias Walter

University of Heidelberg, Kirchhoff Institute for Physics, Im Neuenheimer Feld 227, D-69120?Heidelberg

Frieder H. Loesel

20/10 Perfect Vision, Am Taubenfeld 21/1, D-69123?Heidelberg, Germany

Josef F. Bille

University of Heidelberg, Kirchhoff Institute for Physics, Im Neuenheimer Feld 227, D-69120?Heidelberg

Meng Han, Leander Zickler

University of Heidelberg, Kirchhoff Institute for Physics, Im Neuenheimer Feld 227, D-69120?Heidelberg, Germany E-mail: mhan@urz.uni-heidelberg.de

J. Biomed. Opt. 9(4), 760-766 (Jul 01, 2004). doi:10.1117/1.1756919
History: Received Jul. 17, 2003; Revised Oct. 24, 2003; Accepted Nov. 12, 2003; Online July 12, 2004
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Nonlinear laser scanning microscopy is widely used for noninvasive imaging in cell biology and tissue physiology. However, multiphoton fluorescence imaging of dense, transparent connective tissue (e.g., cornea) is challenging since sophisticated labeling or slicing is necessary. High-resolution, high-contrast second harmonic generation (SHG) imaging of corneal tissue based on the intrinsic structure of collagen is discussed. The three-dimensional corneal ultrastructure in depths up to hundreds of microns can be probed noninvasively, without any staining or mechanical slicing. As an important application of second harmonic imaging in ophthalmology, the modification of corneal ultrastructure using femtosecond laser intrastromal ablation is systematically investigated to evaluate next-generation refractive surgical approaches. © 2004 Society of Photo-Optical Instrumentation Engineers.

Figures in this Article
© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Guenter Giese ; Matthias Walter ; Frieder H. Loesel ; Josef F. Bille ; Meng Han, et al.
"Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation", J. Biomed. Opt. 9(4), 760-766 (Jul 01, 2004). ; http://dx.doi.org/10.1117/1.1756919


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