0
Research Papers

Parallel single molecule detection with a fully integrated single-photon 2×2 CMOS detector array

[+] Author Affiliations
Michael Go¨sch

Karolinska Institute, Department of Medical Biochemistry and Biophysics, S-171?77 Stockholm, Sweden

E´cole Polytechnique Fe´de´rale de Lausanne, Laboratoire d’Optique Biomedicale, CH-1015 Lausanne, Switzerland

Alexandre Serov, Tiemo Anhut, Theo Lasser

E´cole Polytechnique Fe´de´rale de Lausanne Laboratoire d"Optique Biomedicale CH-1015 Lausanne, Switzerland

Alexis Rochas, Pierre-Andre´ Besse, Radivoje S. Popovic

E´cole Polytechnique Fe´de´rale de Lausanne, Institute of Microelectronics and Microsystems, CH-1015 Lausanne, Switzerland

Hans Blom

Royal Institute of Technology, Department of Microelectronics and Information Technology, Electrum 229, S-16440?Kista, Sweden

Rudolf Rigler

Karolinska Institute, Department of Medical Biochemistry and Biophysics, SE-171 77 Stockholm, Sweden E-mail: rudolf.rigler@mbb.ki.se

J. Biomed. Opt. 9(5), 913-921 (Sep 01, 2004). doi:10.1117/1.1781668
History: Received Aug. 11, 2003; Revised Jan. 27, 2004; Accepted Feb. 17, 2004; Online September 17, 2004
Text Size: A A A

We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2×2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an actively quenched avalanche photodiode (APD)] by measurements on free diffusing molecules at different concentrations. Despite the order of magnitude lower detection efficiency of the CMOS detector compared to the state-of-the-art single-photon detecting module, we achieve single molecule sensitivity and reliably determine molecule concentrations. In addition, the CMOS detector performance for the determination of the fraction of slowly diffusing molecules in a primer solution (two-component analysis) is demonstrated. The potential of this new technique for high-throughput confocal-detection-based systems is discussed. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Michael Go¨sch ; Alexandre Serov ; Tiemo Anhut ; Theo Lasser ; Alexis Rochas, et al.
"Parallel single molecule detection with a fully integrated single-photon 2×2 CMOS detector array", J. Biomed. Opt. 9(5), 913-921 (Sep 01, 2004). ; http://dx.doi.org/10.1117/1.1781668


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In