Research Papers

Two-photon laser scanning fluorescence microscopy using photonic crystal fiber

[+] Author Affiliations
Gail McConnell

University of Strathclyde, Centre for Biophotonics, SIBS, 27 Taylor Street, Glasgow?G4?0NR, Scotland

Erling Riis

University of Strathclyde, Department of Physics, John Anderson Building, Glasgow?G4?0NG, Scotland E-mail: g.mcconnell@strath.ac.uk

J. Biomed. Opt. 9(5), 922-927 (Sep 01, 2004). doi:10.1117/1.1778734
History: Received Sep. 3, 2003; Revised Jan. 8, 2004; Revised Feb. 5, 2004; Accepted Feb. 16, 2004; Online September 17, 2004
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We report the application of a simple yet powerful modular pulse compression system based on photonic crystal fibers that improves on incumbent two-photon laser scanning fluorescence microscopy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of IR radiaton of less than 35 fs duration reaching the sample. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Gail McConnell and Erling Riis
"Two-photon laser scanning fluorescence microscopy using photonic crystal fiber", J. Biomed. Opt. 9(5), 922-927 (Sep 01, 2004). ; http://dx.doi.org/10.1117/1.1778734


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