Research Papers

Blood cell counting and classification by nonflowing laser light scattering method

[+] Author Affiliations
Ye Yang, Zhenxi Zhang, Xinhui Yang

Xi’an Jiaotong University, Institute of Biomedical Engineering, Xi’an?710049, China E-mail: ye.yang@umassmed.edu

Joon Hock Yeo

Nanyang Technological University, School of MPE, Nanyang Avenue, Singapore?639798

LiJun Jiang

Institute for Infocom Research (I2R), 21 Heng Mui Keng Terrace, Singapore?119613

Dazong Jiang

Xi’an Jiaotong University, Institute of Biomedical Engineering, Xi’an?710049, China

J. Biomed. Opt. 9(5), 995-1001 (Sep 01, 2004). doi:10.1117/1.1782572
History: Received Dec. 30, 2002; Revised Nov. 11, 2003; Revised Jan. 8, 2004; Accepted Jan. 16, 2004; Online September 17, 2004
Text Size: A A A

We present a nonflowing laser light scattering method for automatically counting and classifying blood cells. A linear charge-coupled device (CCD) and a silicon photoelectric cell (which is placed behind a pinhole plate on the CCD) form a double-detector structure: the CCD is used to detect the scattered light intensity distribution of the blood cells and the silicon photoelectric cell to complete the focusing process. An isotropic sphere, with relative refractivity near 1, is used to model the blood cell. Mie theory is used to describe the scattering of white blood cells and platelets, and anomalous diffraction, red blood cells. To obtain the size distribution of blood cells from their scattered light intensity distribution, the nonnegative constraint least-squares (NNLS) method combined with the Powell method and the precision punishment method are used. Both numerical simulation and experimental results are presented. This method can be used not only to measure the mean and the distribution of red blood cell size, but also to divide the white blood cells into three classes: lymphocytes, middle-sized cells, and neutrocytes. The experimental results show a linear relationship between the blood cell (both white and red blood cells) concentration and the scattered light intensity, and therefore, the number of blood cells in a unit volume can be determined from this relationship. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Ye Yang ; Zhenxi Zhang ; Xinhui Yang ; Joon Hock Yeo ; LiJun Jiang, et al.
"Blood cell counting and classification by nonflowing laser light scattering method", J. Biomed. Opt. 9(5), 995-1001 (Sep 01, 2004). ; http://dx.doi.org/10.1117/1.1782572


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement

 

 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.