Research Papers

In vivo assessment of thermal damage in the liver using optical spectroscopy

[+] Author Affiliations
Clay R. Buttemere

Vanderbilt University, Department of Biomedical Engineering, Nashville, Tennessee?37235

Ravi S. Chari, Christopher D. Anderson

Vanderbilt University Medical Center, Department of Surgery, Nashville, Tennessee?37235

M. Kay Washington

Vanderbilt University Medical Center, Department of Medical Pathology, Nashville, Tennessee?37235

Anita Mahadevan-Jansen, Wei-Chiang Lin

Vanderbilt University, Department of Biomedical Engineering, Nashville, Tennessee?37235 E-mail: wei-chiang.lin@vanderbilt.edu

J. Biomed. Opt. 9(5), 1018-1027 (Sep 01, 2004). doi:10.1117/1.1779627
History: Received Apr. 22, 2003; Revised Aug. 5, 2003; Accepted Jan. 30, 2004; Online September 17, 2004
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Resection is not a viable treatment option for the majority of liver cancer patients. Alternatives to resection include thermotherapies such as radio-frequency ablation; however, these therapies lack adequate intraoperative feedback regarding the degree and margins of tissue thermal damage. In this proof of principle study, we test the ability of fluorescence and diffuse reflectance spectroscopy to assess local thermal damage in vivo. Spectra were acquired in vivo from healthy canine liver tissue undergoing radio-frequency ablation using a portable fiber-optic-based spectroscopic system. The major observed spectral alterations on thermal coagulation were a red shift in the fluorescence emission peak at 480 nm, a decrease in the overall fluorescence intensity, and an increase in the diffuse reflectance from 450 to 750 nm. Spectral changes were quantified and correlated to tissue histology. We found a good correlation between the proposed spectral correlates of thermal damage and histology. The results of this study suggest that fluorescence and diffuse reflectance spectroscopy show strong potential as tools to monitor liver tissue thermal damage intraoperatively. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Clay R. Buttemere ; Ravi S. Chari ; Christopher D. Anderson ; M. Kay Washington ; Anita Mahadevan-Jansen, et al.
"In vivo assessment of thermal damage in the liver using optical spectroscopy", J. Biomed. Opt. 9(5), 1018-1027 (Sep 01, 2004). ; http://dx.doi.org/10.1117/1.1779627


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