Research Papers

Raman spectroscopy of in vivo cutaneous melanin

[+] Author Affiliations
Zhiwei Huang

British Columbia Cancer Research Centre, Cancer Imaging Department, 601 West 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada

Harvey Lui

University of British Columbia, Division of Dermatology and Vancouver Coastal Health Research Institute, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, Canada

X. K. Chen

Simon Fraser University, Department of Physics, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada

Abdulmajeed Alajlan, David I. McLean

University of British Columbia, Division of Dermatology and Vancouver Coastal Health Research Institute, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, Canada

Haishan Zeng

British Columbia Cancer Research Centre, Cancer Imaging Department, 601 West 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada E-mail: hzeng@bccrc.ca

J. Biomed. Opt. 9(6), 1198-1205 (Nov 01, 2004). doi:10.1117/1.1805553
History: Received Oct. 8, 2003; Revised Mar. 11, 2004; Accepted Mar. 16, 2004; Online November 22, 2004
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We successfully acquire the in vivo Raman spectrum of melanin from human skin using a rapid near-infrared (NIR) Raman spectrometer. The Raman signals of in vivo cutaneous melanin are similar to those observed from natural and synthetic eumelanins. The melanin Raman spectrum is dominated by two intense and broad peaks at about 1580 and 1380 cm−1, which can be interpreted as originating from the in-plane stretching of the aromatic rings and the linear stretching of the C-C bonds within the rings, along with some contributions from the C-H vibrations in the methyl and methylene groups. Variations in the peak frequencies and bandwidths of these two Raman signals due to differing biological environments have been observed in melanin from different sources. The ability to acquire these unique in vivo melanin signals suggests that Raman spectroscopy may be a useful clinical method for noninvasive in situ analysis and diagnosis of the skin. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Zhiwei Huang ; Harvey Lui ; X. K. Chen ; Abdulmajeed Alajlan ; David I. McLean, et al.
"Raman spectroscopy of in vivo cutaneous melanin", J. Biomed. Opt. 9(6), 1198-1205 (Nov 01, 2004). ; http://dx.doi.org/10.1117/1.1805553


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