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Research Papers

In vivo measurement of time-resolved autofluorescence at the human fundus

[+] Author Affiliations
Dietrich Schweitzer, Martin Hammer, Frank Schweitzer, Roswitha Anders, Torsten Doebbecke, Stefan Schenke

University of Jena, Department of Experimental Ophthalmology, Bachstr. 18, 07740 Jena, Germany E-mail: Dietrich.Schweitzer@med.uni-jena.de

E. R. Gaillard

Northern Illinois University, Department of Chemistry/Biochemistry, Normal Road/Faraday Hall 324, DeKalb, Il 60115, USA E-mail: Gillard.@uni.edu

J. Biomed. Opt. 9(6), 1214-1222 (Nov 01, 2004). doi:10.1117/1.1806833
History: Received Sep. 22, 2003; Revised Feb. 24, 2004; Accepted Apr. 18, 2004; Online November 22, 2004
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An experimental setup for measurement of time-resolved autofluorescence of the human eye fundus is demonstrated. The method combines laser scanning technique and time-correlated single photon counting. The light source is a laser diode, delivering pulses of about 100 ps duration at a repetition rate of 40 MHz. The excitation wavelength is 446 nm and the cutoff wavelength of fluorescence detection is at 475 nm. The autofluorescence can be determined with a spatial resolution of 80×80 μm2 and 25 ps time resolution. The fluorescence decay is optimally approximated by a biexponential model. The dominating lifetime τ1 is shortest in the macula (320 to 380 ps) and reaches 1500 ps in the optic disk. The lifetime τ2 varies between 2 ns and 5 ns, but the spatial distribution is more homogeneous. Respiration of 100% oxygen for 6 min leads to changes in the fluorescence lifetime pointing to detection of coenzymes. Diagrams of lifetime τ2 versus τ1 are well suited for comparison of substances. Such lifetime clusters of a 20 deg macular field of a young healthy subject and of a patient suffering from dry age-related macular degeneration overlap only partially with τ2-τ1 clusters of lipofuscin. © 2004 Society of Photo-Optical Instrumentation Engineers.

© 2004 Society of Photo-Optical Instrumentation Engineers

Citation

Dietrich Schweitzer ; Martin Hammer ; Frank Schweitzer ; Roswitha Anders ; Torsten Doebbecke, et al.
"In vivo measurement of time-resolved autofluorescence at the human fundus", J. Biomed. Opt. 9(6), 1214-1222 (Nov 01, 2004). ; http://dx.doi.org/10.1117/1.1806833


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