Special Section on Optics in Neuroscience

Imaging ex vivo healthy and pathological human brain tissue with ultra-high-resolution optical coherence tomography

[+] Author Affiliations
Kostadinka Bizheva, Angelika Unterhuber, Boris Hermann, Boris Povazˇay, Harald Sattmann, A. F. Fercher

Medical University of Vienna and Christian Doppler Laboratory, Center for Biomedical Engineering and Physics, Vienna A-1090, Austria

Wolfgang Drexler

Medical University of Vienna and Christian Doppler Laboratory, Center for Biomedical Engineering and Physics, Waehringer Strasse 13, Vienna A-1090, Austria E-mail: Wolfgang.Drexler@meduniwler@ac.at

Matthias Preusser, Herbert Budka

Medical University of Vienna and Vienna General Hospital, Institute of Neurology, Vienna A-1090, Austria

Andreas Stingl, Tuan Le

FEMTOLASERS GmbH, Fernkorngasse 10, Vienna A-1100, Austria

J. Biomed. Opt. 10(1), 011006 (Jan. 31, 2005). doi:10.1117/1.1851513
History: Received Feb. 20, 2004; Revised Jul. 1, 2004; Accepted Aug. 8, 2004; Jan. 31, 2005; Online January 31, 2005
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The ability of ultra-high-resolution optical coherence tomography (UHR OCT) to discriminate between healthy and pathological human brain tissue is examined by imaging ex vivo tissue morphology of various brain biopsies. Micrometer-scale OCT resolution (0.9×2 μm, axial×lateral) is achieved in biological tissue by interfacing a state-of-the-art Ti:Al2O3 laser (λc=800nm, Δλ=260 nm, and Pout=120mW exfiber) to a free-space OCT system utilizing dynamic focusing. UHR OCT images are acquired from both healthy brain tissue and various types of brain tumors including fibrous, athypical, and transitional meningioma and ganglioglioma. A comparison of the tomograms with standard hematoxylin and eosin (H&E) stained histological sections of the imaged biopsies demonstrates the ability of UHR OCT to visualize and identify morphological features such as microcalcifications (>20 μm), enlarged nuclei of tumor cells (∼8 to 15 μm), small cysts, and blood vessels, which are characteristic of neuropathologies and normally absent in healthy brain tissue. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Kostadinka Bizheva ; Angelika Unterhuber ; Boris Hermann ; Boris Povazˇay ; Harald Sattmann, et al.
"Imaging ex vivo healthy and pathological human brain tissue with ultra-high-resolution optical coherence tomography", J. Biomed. Opt. 10(1), 011006 (Jan. 31, 2005). ; http://dx.doi.org/10.1117/1.1851513


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