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Research Papers

Broadband reflectance measurements of light penetration, blood oxygenation, hemoglobin concentration, and drug concentration in human intraperitoneal tissues before and after photodynamic therapy

[+] Author Affiliations
Hsing-Wen Wang

University of Pennsylvania, Department of Physics and Astronomy, 209 South 33rd Street, Philadelphia, Pennsylvania 19104-6396

University of Pennsylvania, Department of Radiation Oncology, 3620 Hamilton Walk, Philadelphia, Pennsylvania 19104-6072

Timothy C. Zhu

University of Pennsylvania, Department of Radiation Oncology, 3620 Hamilton Walk, Philadelphia, Pennsylvania 19104-6072

Mary E. Putt

University of Pennsylvania, Department of Biostatistics and Epidemiology, 423 Guardian Drive Philadelphia, Pennsylvania 19104-4209

Michael Solonenko

University of Pennsylvania, Department of Physics and Astronomy, 209 South 33rd Street, Philadelphia, Pennsylvania 19104-6396

James Metz, Andreea Dimofte, Jeremy Miles

University of Pennsylvania, Department of Radiation Oncology, 3620 Hamilton Walk, Philadelphia, Pennsylvania 19104-6072

Douglas L. Fraker

University of Pennsylvania, Department of Surgery, 3400 Spruce Street, 4 Silverstein Philadelphia, Pennsylvania 19104

Eli Glatstein, Stephen M. Hahn

University of Pennsylvania, Department of Radiation Oncology, 3400 Spruce Street, 2 Donner, Philadelphia, Pennsylvania 19104-4283

Arjun G. Yodh

University of Pennsylvania, Department of Physics and Astronomy, 209 South 33rd Street, Philadelphia, Pennsylvania 19104-6396

University of Pennsylvania, Department of Radiation Oncology, 3620 Hamilton Walk, Philadelphia, Pennsylvania 19104-6072

J. Biomed. Opt. 10(1), 014004 (Feb. 10, 2005). doi:10.1117/1.1854679
History: Received Mar. 15, 2004; Revised Jun. 21, 2004; Accepted Jun. 28, 2004; Feb. 10, 2005; Online February 10, 2005
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We evaluate Photofrin-mediated photodynamic therapy (PDT) in a phase 2 clinical trial as an adjuvant to surgery to treat peritoneal carcinomatosis. We extract tissue optical [reduced scattering (μs), absorption (μa), and attenuation coefficients (μeff)] and physiological [blood oxygen saturation (%StO2), total hemoglobin concentration (THC), and photosensitizer concentration (cPhotofrin)] properties in 12 patients using a diffuse reflectance instrument and algorithms based on the diffusion equation. Before PDT, in normal intraperitoneal tissues %StO2 and THC ranged between 32 to 100% and 19 to 263 μM, respectively; corresponding data from tumor tissues ranged between 11 to 44% and 61 to 224 μM. Tumor %StO2 is significantly lower than oxygenation of normal intraperitoneal tissues in the same patients. The mean (±standard error of mean) penetration depth (δ) in millimeters at 630 nm is 4.8(±0.6) for small bowel, 5.2 (±0.67) for large bowel, 3.39(±0.29) for peritoneum, 5.19(±1.4) for skin, 1.0(±0.1) for liver, and 3.02(±0.66) for tumor. cPhotofrin in micromolars is 4.9(±2.3) for small bowel, 4.8(±2.3) for large bowel, 3.0 (±1.0) for peritoneum, 2.5(±0.9) for skin, and 7.4(±2.8) for tumor. In all tissues examined, mean cPhotofrin tends to decrease after PDT, perhaps due to photobleaching. These results provide benchmark in-vivo tissue optical property data, and demonstrate the feasibility of in-situ measurements during clinical PDT treatments. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Topics

Tissues

Citation

Hsing-Wen Wang ; Timothy C. Zhu ; Mary E. Putt ; Michael Solonenko ; James Metz, et al.
"Broadband reflectance measurements of light penetration, blood oxygenation, hemoglobin concentration, and drug concentration in human intraperitoneal tissues before and after photodynamic therapy", J. Biomed. Opt. 10(1), 014004 (Feb. 10, 2005). ; http://dx.doi.org/10.1117/1.1854679


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