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Research Papers

Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy

[+] Author Affiliations
Katja Schenke-Layland

Children’s Hospital Los Angeles, Saban Research Institute, 4650 Sunset Boulevard, MS#137, Los Angeles, California 90027 E-mail: b7sckt@web.de

Iris Riemann

Fraunhofer Institute of Biomedical Technology, 66386 St. Ingbert, Germany

Ulrich A. Stock

Humboldt University, University Hospital Charite´, Department of Medical Physics, and Biophysics, 10098 Berlin, Germany

Karsten Ko¨nig

Fraunhofer Institute of Biomedical Technology, 66386 St. Ingbert, Germany

J. Biomed. Opt. 10(2), 024017 (Apr. 29, 2005). doi:10.1117/1.1896966
History: Received Mar. 15, 2004; Revised Aug. 12, 2004; Accepted Aug. 20, 2004; Apr. 29, 2005; Online April 29, 2005
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Multiphoton imaging represents a novel and very promising medical diagnostic technology for the high-resolution analysis of living biological tissues. We performed multiphoton imaging to analyzed structural features of extracellular matrix (ECM) components, e.g., collagen and elastin, of vital pulmonary and aortic heart valves. High-resolution autofluorescence images of collagenous and elastic fibers were demonstrated using multifluorophore, multiphoton excitation at two different wavelengths and optical sectioning, without the requirement of embedding, fixation, or staining. Collagenous structures were selectively imaged by detection of second harmonic generation (SHG). Additionally, routine histology and electron microscopy were integrated to verify the observed results. In comparison with pulmonary tissues, aortic heart valve specimens show very similar matrix formations. The quality of the resulting three-dimensional (3-D) images enabled the differentiation between collagenous and elastic fibers. These experimental results indicate that multiphoton imaging with near-infrared (NIR) femtosecond laser pulses may prove to be a useful tool for the nondestructive monitoring and characterization of cardiovascular structures. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Katja Schenke-Layland ; Iris Riemann ; Ulrich A. Stock and Karsten Ko¨nig
"Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy", J. Biomed. Opt. 10(2), 024017 (Apr. 29, 2005). ; http://dx.doi.org/10.1117/1.1896966


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