Research Papers

Raman scattering properties of human pterygium tissue

[+] Author Affiliations
Aiguo Shen, Yong Ye, Xiaohua Wang, Changchun Chen

Wuhan University, College of Chemistry and Molecular Sciences, Wuhan, 430072, China

Hanbin Zhang

Wuhan AIER Eye Hospital, Wuhan, 430063, China

Jiming Hu

Wuhan University, College of Chemistry and Molecular Sciences, Wuhan 430072, China E-mail: jmhu@whu.edu.cn

J. Biomed. Opt. 10(2), 024036 (Apr. 13, 2005). doi:10.1117/1.1888345
History: Received Jul. 26, 2004; Revised Oct. 16, 2004; Accepted Nov. 2, 2004; Apr. 13, 2005; Online April 13, 2005
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Pterygia, caused by fibrovascular growth of conjunctiva, are a common ophthalmic disease. However, the molecular composition of pterygium tissue has not been completely understood, and therefore the aim of this study is to investigate the spectroscopic differences between normal human bulbar conjunctiva and human pterygium tissue using a confocal Raman system. The high signal-to-noise ratio spectra from pterygium and conjunctiva tissue were obtained by this technique without any sample preparation and the time of detection required less than 3 min. Comparing Raman spectra of two types of tissue, there are obvious changes, including intensity decrease at ∼1585 cm−1 and intensity increase at ∼1748, 1156, and 1521 cm−1 with the lesion of conjunctiva. Additionally, the amide I vibrational mode of proteins in conjunctiva is significantly different than that in pterygium. The use of pathology, immunology, and the peroxidation of the lipids in conjunction with the Raman results indicate that the presence of additional elastic fibers, mast cells, and lymphocytes in pterygium, as compared with normal bulbar conjunctiva, have fewer unsaturated fat acids. The present study demonstrates that Raman spectroscopy can be potentially applied to diagnose pterygia clinically. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Aiguo Shen ; Yong Ye ; Xiaohua Wang ; Changchun Chen ; Hanbin Zhang, et al.
"Raman scattering properties of human pterygium tissue", J. Biomed. Opt. 10(2), 024036 (Apr. 13, 2005). ; http://dx.doi.org/10.1117/1.1888345


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