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Research Papers

Functional imaging of dye concentration in tissue phantoms by spectroscopic optical coherence tomography

[+] Author Affiliations
Trude Støren

Norwegian University of Science and Technology, Department of Physics, N-7491 Trondheim, Norway

Arne Røyset

SINTEF Materials Technology, Department of Applied Physics, N-7465 Trondheim, Norway

Lars O. Svaasand

Norwegian University of Science and Technology, Department of Electronics and Telecommunications, N-7491 Trondheim, Norway

Tore Lindmo

Norwegian University of Science and Technology, Department of Physics, N-7491 Trondheim, Norway E-mail: Tore.Lindmo@phys.ntnu.no

J. Biomed. Opt. 10(2), 024037 (Mar. 5, 2004Aug. 12, 2004Aug. 17, 2004Apr. 29, 2005April 29, 2005). doi:10.1117/1.1898242
History: Received Mar. 5, 2004; Revised Aug. 12, 2004; Accepted Aug. 17, 2004; Apr. 29, 2005; Online April 29, 2005
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We present functional imaging of the concentration of a photodynamic therapy (PDT)-related dye in scattering tissue phantoms based on spatially resolved measurements of optical properties through spectroscopic optical coherence tomography (OCT). Expressions for the OCT signal are developed, enabling estimation of depth-resolved sample optical properties. Based on these expressions, we discuss speckle statistics and speckle correlations of the OCT signal. Speckle noise reduction is performed by spatial filtering and is used to improve accuracy in the estimated optical properties at the expense of spatial resolution. An analytic expression for the precision in the estimated optical properties is derived. This expression shows that axial filtering, and thereby a reduction of axial resolution, gives a larger improvement in precision compared to the same filtering and reduction in the transversal resolution. It also shows that imaging with a shorter coherence length, or a larger numerical aperture, improves precision when the filter length determines the spatial resolution. Good agreement is obtained between experimentally determined and theoretically predicted variance in the estimated attenuation coefficients and dye concentration. Finally, we present guidelines for spectroscopic OCT systems for concentration imaging and discuss application of the method to more realistic phantoms and tissue. © 2005 Society of Photo-Optical Instrumentation Engineers.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Trude Støren ; Arne Røyset ; Lars O. Svaasand and Tore Lindmo
"Functional imaging of dye concentration in tissue phantoms by spectroscopic optical coherence tomography", J. Biomed. Opt. 10(2), 024037 (Mar. 5, 2004Aug. 12, 2004Aug. 17, 2004Apr. 29, 2005April 29, 2005). ; http://dx.doi.org/10.1117/1.1898242


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