Modern vibrational spectroscopy is a powerful tool for biomedical applications. The biochemical information content is high and there is a vast body of literature connecting spectral features to specific molecular structures and environments. Infrared and Raman spectra contain many bands that can be used to identify molecules and, in some cases, to measure the composition of complex, multi-component samples. The positions and relative intensities of various spectral bands can also be used to probe the primary, secondary, tertiary, and quaternary structure of biopolymers. There is now a large body of biophysical/biochemical literature connecting spectral features with molecular structure and environment and a specialized tissue literature that builds on this. Equally importantly, modern instruments are compact, reliable, and easy to use and are supported by powerful software that enables extraction of even subtle features and correlations.
© 2005 Society of Photo-Optical Instrumentation Engineers
Michael D. Morris ; Andrew Berger and Anita Mahadevan-Jansen
"Infrared and Raman Spectroscopy", J. Biomed. Opt. 10(3), 031101 (May 23, 2005). ; http://dx.doi.org/10.1117/1.1906246