Special Section on Infrared and Raman Spectroscopy

Analysis of biofluids in aqueous environment based on mid-infrared spectroscopy

[+] Author Affiliations
Heinz Fabian, Peter Lasch, Dieter Naumann

Robert Koch-Institute, P13, Nordufer 20, 13353 Berlin, Germany E-mail: naumannd@rki.de

J. Biomed. Opt. 10(3), 031103 (May 24, 2005). doi:10.1117/1.1917844
History: Received Aug. 31, 2004; Revised Oct. 1, 2004; Accepted Oct. 1, 2004; May 24, 2005; Online May 24, 2005
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In this study we describe a semiautomatic Fourier transform infrared spectroscopic methodology for the analysis of liquid serum samples, which combines simple sample introduction with high sample throughput. The applicability of this new infrared technology to the analysis of liquid serum samples from a cohort of cattle naturally infected with bovine spongiform encephalopathy and from controls was explored in comparison to the conventional approach based on transmission infrared spectroscopy of dried serum films. Artifical neural network analysis of the infrared data was performed to differentiate between bovine spongiform encephalopathy-negative controls and animals in the late stage of the disease. After training of artifical neural network classifiers, infrared spectra of sera from an independent external validation data set were analyzed. In this way, sensitivities between 90 and 96% and specificities between 84 and 92% were achieved, respectively, depending upon the strategy of data collection and data analysis. Based on these results, the advantages and limitations of the liquid sample technique and the dried film approach for routine analysis of biofluids are discussed. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Heinz Fabian ; Peter Lasch and Dieter Naumann
"Analysis of biofluids in aqueous environment based on mid-infrared spectroscopy", J. Biomed. Opt. 10(3), 031103 (May 24, 2005). ; http://dx.doi.org/10.1117/1.1917844


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