0
Special Section on Infrared and Raman Spectroscopy

Glucose determination in human aqueous humor with Raman spectroscopy

[+] Author Affiliations
James L. Lambert, Christine C. Pelletier

Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California 91109-8099

Mark Borchert

University of Southern California, Division of Ophthalmology, Children Hospital Los Angeles and Department of Ophthalmology, Keck School of Medicine, Los Angeles, California

J. Biomed. Opt. 10(3), 031110 (Jun. 7, 2005). doi:10.1117/1.1914843
History: Received Aug. 3, 2004; Revised Oct. 4, 2004; Accepted Oct. 5, 2004; Jun. 7, 2005; Online June 07, 2005
Text Size: A A A

It has been suggested that spectroscopic analysis of the aqueous humor of the eye could be used to indirectly predict blood glucose levels in diabetics noninvasively. We have been investigating this potential using Raman spectroscopy in combination with partial least squares (PLS) analysis. We have determined that glucose at clinically relevant concentrations can be accurately predicted in human aqueous humor in vitro using a PLS model based on artificial aqueous humor. We have further determined that with proper instrument design, the light energy necessary to achieve clinically acceptable prediction of glucose does not damage the retinas of rabbits and can be delivered at powers below internationally acceptable safety limits. Herein we summarize our current results and address our strategies to improve instrument design. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

James L. Lambert ; Christine C. Pelletier and Mark Borchert
"Glucose determination in human aqueous humor with Raman spectroscopy", J. Biomed. Opt. 10(3), 031110 (Jun. 7, 2005). ; http://dx.doi.org/10.1117/1.1914843


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In