Special Section on Infrared and Raman Spectroscopy

Raman spectroscopy study of atherosclerosis in human carotid artery

[+] Author Affiliations
Grazielle V. Nogueira, Landulfo Silveira, Airton A. Martin, Renato A. Za⁁ngaro, Marcos T. T. Pacheco

University of Vale do Parai´ba-UNIVAP, Institute of Research and Development, Av. Shishima Hifumi, 2911, Sa˜o Jose´ dos Campos, SP, Brazil, 12244-000

Maria C. Chavantes, Carlos A. Pasqualucci

University of Sa˜o Paulo, Department of Cardiovascular Pathology, Av. Dr. Arnaldo, 455, Sa˜o Paulo, SP, Brazil, 01246-903

J. Biomed. Opt. 10(3), 031117 (May 23, 2005). doi:10.1117/1.1908129
History: Received Jun. 22, 2004; Revised Jan. 18, 2005; Accepted Jan. 18, 2005; May 23, 2005; Online May 23, 2005
Text Size: A A A

Fourier-transform (FT)-Raman spectroscopy has been used for identification and evaluation of human artherosclerotic lesions, providing biochemical information on arteries. In this work, fragments of human carotid arteries postmortem were analyzed using a FT-Raman spectrometer operating at an excitation wavelength of 1064 nm, power of 200 mW, and spectral resolution of 4cm1. A total of 75 carotid fragments were spectroscopically scanned and FT-Raman results were compared with histopathology. Discriminant analysis using Mahalanobis distance was applied over principal components scores for tissue classification into three categories: nonatherosclerotic, atherosclerotic plaque without calcification and with calcification. Nonatherosclerotic artery, atherosclerotic plaque, and calcified plaque exhibit spectral signatures related to biochemicals presented in each tissue type, such as bands of collagen and elastin (proteins), cholesterol and its esters, and calcium hydroxyapatite and carbonate apatite, respectively. Spectra of nonatherosclerotic artery were then classified into two groups: normal and discrete diffuse thickening of the intima layer (first group) and moderate and intense diffuse thickening of the intima layer (second group). FT-Raman could identify and classify the tissues found in the atherosclerotic process in human carotid in vitro and had the ability to identify alterations to the diffuse thickening of the intima layer and classify it depending on the intensity of the thickening. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Grazielle V. Nogueira ; Landulfo Silveira, Jr. ; Airton A. Martin ; Renato A. Za⁁ngaro ; Marcos T. T. Pacheco, et al.
"Raman spectroscopy study of atherosclerosis in human carotid artery", J. Biomed. Opt. 10(3), 031117 (May 23, 2005). ; http://dx.doi.org/10.1117/1.1908129


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.