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Special Section on Infrared and Raman Spectroscopy

Ex vivo detection and characterization of early dental caries by optical coherence tomography and Raman spectroscopy

[+] Author Affiliations
Alex C.-T. Ko, Lin-P’ing Choo-Smith, Mark Hewko, Lorenzo Leonardi, Michael G. Sowa

National Research Council Canada, Institute for Biodiagnostics, 435 Ellice Ave., Winnipeg MB, Canada R3B 1Y6

Cecilia C. S. Dong, Peter Williams

University of Manitoba, Department of Restorative Dentistry, Faculty of Dentistry, 780 Bannatyne Ave., Winnipeg MB, Canada R3E 0W2

Blaine Cleghorn

Dalhousie University, Department of Dental Clinic Sciences, Faculty of Dentistry, 5981 University Ave., Halifax NS, Canada B3H 3J5

J. Biomed. Opt. 10(3), 031118 (May 26, 2005). doi:10.1117/1.1915488
History: Received Aug. 12, 2004; Revised Dec. 9, 2004; Accepted Dec. 9, 2004; May 26, 2005; Online May 26, 2005
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Early dental caries detection will facilitate implementation of nonsurgical methods for arresting caries progression and promoting tooth remineralization. We present a method that combines optical coherence tomography (OCT) and Raman spectroscopy to provide morphological information and biochemical specificity for detecting and characterizing incipient carious lesions found in extracted human teeth. OCT imaging of tooth samples demonstrated increased light backscattering intensity at sites of carious lesions as compared to the sound enamel. The observed lesion depth on an OCT image was approximately 290 μm matching those previously documented for incipient caries. Using Raman microspectroscopy and fiber-optic-based Raman spectroscopy to characterize the caries further, spectral changes were observed in PO43 vibrations arising from hydroxyapatite of mineralized tooth tissue. Examination of various ratios of PO43ν2,ν3,ν4 vibrations against the ν1 vibration showed consistent increases in carious lesions compared to sound enamel. The changes were attributed to demineralization-induced alterations of enamel crystallite morphology and/or orientation. OCT imaging is useful for screening carious sites and determining lesion depth, with Raman spectroscopy providing biochemical confirmation of caries. The combination has potential for development into a new fiber-optic diagnostic tool enabling dentists to identify early caries lesions with greater sensitivity and specificity. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Alex C.-T. Ko ; Lin-P’ing Choo-Smith ; Mark Hewko ; Lorenzo Leonardi ; Michael G. Sowa, et al.
"Ex vivo detection and characterization of early dental caries by optical coherence tomography and Raman spectroscopy", J. Biomed. Opt. 10(3), 031118 (May 26, 2005). ; http://dx.doi.org/10.1117/1.1915488


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