Research Papers

Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis

[+] Author Affiliations
Shean-Jen Chen, Yuan-Deng Su

National Cheng Kung University, Department of Engineering Science, Tainan 701, Taiwan

Feng-Ming Hsiu, Chia-Yuan Tsou, Yi-Kuang Chen

National Central University, Department of Mechanical Engineering, Chung-Li 320, Taiwan

J. Biomed. Opt. 10(3), 034005 (Jun. 1, 2005). doi:10.1117/1.1924713
History: Received Feb. 26, 2004; Revised Aug. 23, 2004; Accepted Dec. 20, 2004; Jun. 1, 2005; Online June 01, 2005
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Surface plasmon resonance (SPR) phase-shift interferometry (PSI) is a novel technique which combines SPR and modified Mach–Zehnder PSI to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable quantitative information. The SPR-PSI imaging system has an enhanced detection limit of 2.5×107 refraction index change, a long-term phase stability of π/100 in 30 min, and a spatial phase resolution of π/300 with 100×100 μm2 detection area. This study successfully demonstrates the label-free observation of 15-mer DNA microarray. © 2005 Society of Photo-Optical Instrumentation Engineers.

© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Shean-Jen Chen ; Yuan-Deng Su ; Feng-Ming Hsiu ; Chia-Yuan Tsou and Yi-Kuang Chen
"Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis", J. Biomed. Opt. 10(3), 034005 (Jun. 1, 2005). ; http://dx.doi.org/10.1117/1.1924713


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