Special Section on Chemical and Genetic Sensors in Biomedical Research

Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging

[+] Author Affiliations
James R. Mansfield, Kirk W. Gossage, Clifford C. Hoyt, Richard M. Levenson

Cambridge Research and Instrumentation, Inc.,35-B Cabot Road, Woburn, Massachusetts, 01801

J. Biomed. Opt. 10(4), 041207 (August 30, 2005). doi:10.1117/1.2032458
History: Received March 07, 2005; Revised May 11, 2005; Accepted May 23, 2005; Published August 30, 2005
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The ability to image and quantitate fluorescently labeled markers in vivo has generally been limited by autofluorescence of the tissue. Skin, in particular, has a strong autofluorescence signal, particularly when excited in the blue or green wavelengths. Fluorescence labels with emission wavelengths in the near-infrared are more amenable to deep-tissue imaging, because both scattering and autofluorescence are reduced as wavelengths are increased, but even in these spectral regions, autofluorescence can still limit sensitivity. Multispectral imaging (MSI), however, can remove the signal degradation caused by autofluorescence while adding enhanced multiplexing capabilities. While the availability of spectral “libraries” makes multispectral analysis routine for well-characterized samples, new software tools have been developed that greatly simplify the application of MSI to novel specimens.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

James R. Mansfield ; Kirk W. Gossage ; Clifford C. Hoyt and Richard M. Levenson
"Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging", J. Biomed. Opt. 10(4), 041207 (August 30, 2005). ; http://dx.doi.org/10.1117/1.2032458


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