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Research Papers

Validation of in vivo fluorochrome concentrations measured using fluorescence molecular tomography

[+] Author Affiliations
Edward E. Graves

Stanford University School of Medicine, Department of Radiation Oncology, Stanford, California 94305 USA

Doreen Yessayan, Gordon Turner, Ralph Weissleder, Vasilis Ntziachristos

Massachusetts General Hospital, Harvard Medical School, Center for Molecular Imaging Research, Department of Radiology, Charlestown, Massachusetts 02129 USA

J. Biomed. Opt. 10(4), 044019 (August 10, 2005). doi:10.1117/1.1993427
History: Received June 04, 2004; Revised March 30, 2005; Accepted April 04, 2005; Published August 10, 2005
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Fluorescence molecular tomography (FMT) has emerged as a means of quantitatively imaging fluorescent molecular probes in three dimensions in living systems. To assess the accuracy of FMT in vivo, translucent plastic tubes containing a turbid solution with a known concentration of Cy 5.5 fluorescent dye are constructed and implanted subcutaneously in nude mice, simulating the presence of a tumor accumulating a fluorescent molecular reporter. Comparisons between measurements of fluorescent tubes made before and after implantation demonstrate that the accuracy of FMT reported for homogeneous phantoms extends to the in vivo situation. The sensitivity of FMT to background fluorescence is tested by imaging fluorescent tubes in mice injected with Cy 5.5-labeled Annexin V. For small tube fluorochrome concentrations, the presence of background fluorescence results in increases in the reconstructed concentration. This phenomenon is counteracted by applying a simple subtraction correction to the measured fluorescence data. The effects of varying tumor photon absorption are simulated by imaging fluorescent tubes with varying ink concentrations, and are found to be minor. These findings demonstrate the in vivo quantitative accuracy of fluorescence tomography, and encourage further development of this imaging modality as well as application of FMT in molecular imaging studies using fluorescent reporters.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Edward E. Graves ; Doreen Yessayan ; Gordon Turner ; Ralph Weissleder and Vasilis Ntziachristos
"Validation of in vivo fluorochrome concentrations measured using fluorescence molecular tomography", J. Biomed. Opt. 10(4), 044019 (August 10, 2005). ; http://dx.doi.org/10.1117/1.1993427


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